| Autor: |
Nong QD; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.; Biotechnology Global Human Resource Development Program, Division of Advanced Science and Biotechnology, Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan., Mohamad Ishak NS; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.; Biotechnology Global Human Resource Development Program, Division of Advanced Science and Biotechnology, Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan., Matsuura T; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan., Kato Y; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan. kato_yasuhiko@bio.eng.osaka-u.ac.jp.; Frontier Research Base of Global Young Researchers, Graduate School of Engineering, Osaka University, Suita, Japan. kato_yasuhiko@bio.eng.osaka-u.ac.jp., Watanabe H; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan. |
| Abstrakt: |
Sexually dimorphic traits are common and widespread among animals. The expression of the Doublesex-/Mab-3-domain (DM-domain) gene family has been widely studied in model organisms and has been proven to be essential for the development and maintenance of sex-specific traits. However, little is known about the detailed expression patterns in non-model organisms. In the present study, we demonstrated the spatiotemporal expression of the DM-domain gene, doublesex1 (dsx1), in the crustacean Daphnia magna, which parthenogenetically produces males in response to environmental cues. We developed a dsx1 reporter strain to track dsx1 activity in vivo by inserting the mCherry gene into the dsx1 locus using the TALEN-mediated knock-in approach. After confirming dsx1 expression in male-specific traits in juveniles and adults, we performed time-lapse imaging of embryogenesis. Shortly after gastrulation stage, a presumptive primary organiser, named cumulus, first showed male-specific dsx1 expression. This cell mass moved to the posterior growth zone that distributes dsx1-expressing progenitor cells across the body during axial elongation, before embryos start male-specific dsx1 expression in sexually dimorphic structures. The present study demonstrated the sex-specific dsx1 expression in cell populations involved in basal body formation. |
