Development of a new tissue injector for subretinal transplantation of human embryonic stem cell derived retinal pigmented epithelium.

Autor: Fernandes RAB; Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA USA.; Department of Ophthalmology and Visual Sciences, Federal University of São Paulo, Rua Botucatu, 822, São Paulo, SP 04023-062 Brazil., Stefanini FR; Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA USA.; Department of Ophthalmology and Visual Sciences, Federal University of São Paulo, Rua Botucatu, 822, São Paulo, SP 04023-062 Brazil., Falabella P; Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA USA.; Department of Ophthalmology and Visual Sciences, Federal University of São Paulo, Rua Botucatu, 822, São Paulo, SP 04023-062 Brazil., Koss MJ; Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA USA.; Augenzentrum Nymphenburger Hoefe, Herzog Carl Theodor Augenklinik, Munich, Germany., Wells T; Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA USA., Diniz B; Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA USA.; Department of Ophthalmology and Visual Sciences, Federal University of São Paulo, Rua Botucatu, 822, São Paulo, SP 04023-062 Brazil., Ribeiro R; Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA USA.; Department of Ophthalmology and Visual Sciences, Federal University of São Paulo, Rua Botucatu, 822, São Paulo, SP 04023-062 Brazil., Schor P; Department of Ophthalmology and Visual Sciences, Federal University of São Paulo, Rua Botucatu, 822, São Paulo, SP 04023-062 Brazil., Maia M; Department of Ophthalmology and Visual Sciences, Federal University of São Paulo, Rua Botucatu, 822, São Paulo, SP 04023-062 Brazil., Penha FM; Department of Ophthalmology and Visual Sciences, Federal University of São Paulo, Rua Botucatu, 822, São Paulo, SP 04023-062 Brazil.; Fundação Universidade Regional de Blumenau, Blumenau, Santa Catarina Brazil., Hinton DR; Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA USA.; Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA USA., Tai YC; Electrical Engineering, California Institute of Technology, Pasadena, CA USA., Humayun M; Department of Ophthalmology, USC Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA USA.; USC Institute for Biomedical Therapeutics, Keck School of Medicine, University of Southern California, Los Angeles, CA USA.
Jazyk: angličtina
Zdroj: International journal of retina and vitreous [Int J Retina Vitreous] 2017 Oct 30; Vol. 3, pp. 41. Date of Electronic Publication: 2017 Oct 30 (Print Publication: 2017).
DOI: 10.1186/s40942-017-0095-6
Abstrakt: Background: Subretinal cell transplantation is a challenging surgical maneuver. This paper describes the preliminary findings of a new tissue injector for subretinal implantation of an ultrathin non-absorbable substrate seeded with human embryonic stem cell-derived retinal pigment epithelium (hESC-RPE).
Methods: Ultrathin Parylene-C substrates measuring 3.5 mm × 6.0 mm seeded with hESC-RPE (implant referred to as CPCB-RPE1) were implanted into the subretinal space of 12 Yucatan minipigs. Animals were euthanized immediately after the procedure and underwent spectral domain optical coherence tomography (SD-OCT) and histological analysis to assess the subretinal placement of the implant. Evaluation of the hESC-RPE cells seeded on the substrate was carried out before and after implantation using standard cell counting techniques.
Results: The tissue injector delivered the CPCB-RPE1 implant through a 1.5 mm sclerotomy and a 1.0-1.5 mm retinectomy. SD-OCT scans and histological examination revealed that substrates were precisely placed in the subretinal space, and that the hESC-RPE cell monolayer continued to cover the surface of the substrate after the surgical procedure.
Conclusion: This innovative tissue injector was able to efficiently deliver the implant in the subretinal space of Yucatan minipigs, preventing significant hESC-RPE cell loss, minimizing tissue trauma, surgical complications and postoperative inflammation.
Databáze: MEDLINE
Externí odkaz: