| Autor: |
Gourley CR; School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, 99164-7520, WA, USA., Negretti NM; School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, 99164-7520, WA, USA., Konkel ME; School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, 99164-7520, WA, USA. konkel@vetmed.wsu.edu. |
| Abstrakt: |
Accurate repair of DNA damage is crucial to ensure genome stability and cell survival of all organisms. Bile functions as a defensive barrier against intestinal colonization by pathogenic microbes. Campylobacter jejuni, a leading bacterial cause of foodborne illness, possess strategies to mitigate the toxic components of bile. We recently found that growth of C. jejuni in medium with deoxycholate, a component of bile, caused DNA damage consistent with the exposure to reactive oxygen species. We hypothesized that C. jejuni must repair DNA damage caused by reactive oxygen species to restore chromosomal integrity. Our efforts focused on determining the importance of the putative AddAB DNA repair proteins. A C. jejuni addAB mutant demonstrated enhanced sensitivity to deoxycholate and was impaired in DNA double strand break repair. Complementation of the addAB mutant restored resistance to deoxycholate, as well as function of the DNA double strand break repair system. The importance of these findings translated to the natural host, where the AddAB system was found to be required for efficient C. jejuni colonization of the chicken intestine. This research provides new insight into the molecular mechanism utilized by C. jejuni, and possibly other intestinal pathogens, to survive in the presence of bile. |
