Modulating effect of MgO-SiO 2 nanoparticles on immunological and histopathological alterations induced by aflatoxicosis in rats.

Autor: Essa SS; Immune Section, Research Institute for Animal Reproduction, Cairo, Egypt., El-Saied EM; Department of Toxicology, Forensic Medicine and Veterinary Regulations, Faculty of Veterinary Medicine, Cairo University, Egypt., El-Tawil OS; Department of Toxicology, Forensic Medicine and Veterinary Regulations, Faculty of Veterinary Medicine, Cairo University, Egypt., Mahmoud MB; Immune Section, Research Institute for Animal Reproduction, Cairo, Egypt., Abd El-Rahman SS; Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Egypt. Electronic address: saharsamirmah@cu.edu.eg.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2017 Dec 15; Vol. 140, pp. 94-104. Date of Electronic Publication: 2017 Oct 25.
DOI: 10.1016/j.toxicon.2017.10.018
Abstrakt: Introduction: AflatoxinB1 (AFB1) is well-known as a feed borne-hepatotoxic and immunosuppressive mycotoxin. This study was conducted to evaluate the efficacy of nanocomposite magnesium oxide and silicon oxide (MgO-SiO 2 ) in reducing the toxic effects of AFB1on the immunity and histological alterations in liver, spleen and intestine of adult male rats.
Experimental Design: Animals were divided into a control (Gp1) and three experimental groups (Gps); Gp2 received feed contained 200 ppb AFB1, Gp3 received feed contained 200 ppb AFB1 and 0.5 g/kg MgO-SiO 2 nanocomposite. While, rats of Gp4 received feed contained 0.5 g/kg MgO-SiO 2 nano-composite.
Methods: Cellular and humoral immune responses, as well as histopathological examination and caspase-3 expression in liver, spleen, and intestine, were all evaluated. Residual concentration of AFB1was determined in serum, liver and fecal samples. The obtained data were statistically analyzed.
Results: AFB1markedly reduced body weight gain and food and water consumption. Cellular immune response (total and differential leukocytes count, neutrophils' phagocytic activity, lymphocyte transformation, macrophage activity and serum lysozyme activity), serum total protein, and humoral immune response (fractions of protein as estimated by SDS- PAGE electrophoresis) were all severely reduced by AFB1. Moreover, AFB1induced marked histological alterations and apoptosis in liver, spleen, and intestine.
Conclusion: These findings suggested that the nanocomposite MgO-SiO 2 has high affinity to adsorb AFB1 and can effectively modulate its toxicity in rats.
Impact Statement: Nanocomposite MgO-SiO 2 may offer a novel effective and cheap approach for the preventive management of aflatoxicosis in animals.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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