Perivitelline threads in cleavage-stage human embryos: observations using time-lapse imaging.

Autor: Kellam L; CARE Fertility, John Webster House, 6 Lawrence Drive, Nottingham Business Park, Nottingham, NG8 6PZ, UK., Pastorelli LM; CARE Fertility, Amberley House, 9 Queens Road, Tunbridge Wells, Kent TN4 9LL, UK., Bastida AM; CARE Fertility, John Webster House, 6 Lawrence Drive, Nottingham Business Park, Nottingham, NG8 6PZ, UK., Senkbeil A; CARE Fertility, John Webster House, 6 Lawrence Drive, Nottingham Business Park, Nottingham, NG8 6PZ, UK., Montgomery S; CARE Fertility, 108-112 Daisy Bank Road, Victoria Park, Manchester M14 5QH, UK., Fishel S; CARE Fertility, John Webster House, 6 Lawrence Drive, Nottingham Business Park, Nottingham, NG8 6PZ, UK., Campbell A; CARE Fertility, John Webster House, 6 Lawrence Drive, Nottingham Business Park, Nottingham, NG8 6PZ, UK. Electronic address: alison.campbell@carefertility.com.
Jazyk: angličtina
Zdroj: Reproductive biomedicine online [Reprod Biomed Online] 2017 Dec; Vol. 35 (6), pp. 646-656. Date of Electronic Publication: 2017 Sep 28.
DOI: 10.1016/j.rbmo.2017.09.004
Abstrakt: Time-lapse imaging of the human preimplantation embryo in vitro has revealed a transient phenomenon involving the appearance of perivitelline threads, commonly observed at the two-cell stage. These threads span the perivitelline space, arising at the specific area where the cytoplasmic membrane contacts the zona pellucida, before any perivitelline space is formed. The threads persist as the cytoplasmic membrane retracts from the zona pellucida to form the first cleavage furrow. In this observational report, these structures and their incidence are described. A total of 834 time-lapse videos from IVF treatment cycles, one per patient, were retrospectively analysed for perivitelline threads, from pronuclear formation until completion of the first cell cycle. Threads were observed in 56.4% (470/834) of embryos and varied from a single to an array spanning an area of the zona pellcida. A total of 91.9% (432/470) were seen to form after cytoplasmic membrane-zona-pellucida contact. A total of 76.4% (359/470) were visible at the first cleavage furrow; 77% (362/470) were associated with cytoplasmic fragments at the two-cell-stage. Presence or absence of threads did not affect embryo development. This descriptive study is limited; further characterization of these structures is needed to elucidate their potential role in early human embryo development.
(Copyright © 2017 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: