Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil.
| Autor: | Valença Barbosa C; Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil, 4365 Pavilhão Arthur Neiva, Rio de Janeiro, 21.045-900, Brazil., de Jesus Batista R; Departamento de Ginecologia e Obstetrícia, IFF/Fiocruz, Rio de Janeiro, Brasil, Av. Rui Barbosa, 716, Flamengo, Rio de Janeiro, 22250-020, Brazil., Pereira Igreja R; Departamento de Medicina Preventiva da Faculdade de Medicina da UFRJ , Av. Carlos Chagas Filho, 373, Edifício do Centro de Ciências da Saúde Bloco K, 2° andar, Sala 49 - Cidade Universitária - Ilha do Fundão, Rio de Janeiro, 21.941-902, Brazil., d'Avila Levy CM; Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil, 4365 Pavilhão Arthur Neiva, Rio de Janeiro, 21.045-900, Brazil., Werneck de Macedo H; Laboratório de Parasitologia do Departamento de Patologia, Hospital Universitário Antônio Pedro/Universidade Federal Fluminense, Niterói, RJ, Rua Marquês do Paraná, 303. 4º andar, sala 12, Niterói, Rio de Janeiro, 24033 900, Brazil., Carneiro Santos HL; Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz/FIOCRUZ, Av. Brasil, 4365 Pavilhão Arthur Neiva, Rio de Janeiro, 21.045-900, Brazil. helenalucias@ioc.fiocruz.br. |
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| Jazyk: | angličtina |
| Zdroj: | Parasites & vectors [Parasit Vectors] 2017 Oct 25; Vol. 10 (1), pp. 518. Date of Electronic Publication: 2017 Oct 25. |
| DOI: | 10.1186/s13071-017-2458-0 |
| Abstrakt: | Background: Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil. Methods: A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers. Results: Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%). Conclusions: DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro. |
| Databáze: | MEDLINE |
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