Cut-off value for absolute lymphocytes as an alternative for the immunophenotypic analysis of CD3+ T cells in the monitoring of immunosuppressive therapy with thymoglobulin.

Autor:	Furlanetto G; Universidade Feevale., Alegretti AP; Hospital de Clínicas de Porto Alegre., Farias MG; Hospital de Clínicas de Porto Alegre., Freitas PAC; Hospital de Clínicas de Porto Alegre., Lara GM; Universidade Feevale., Pedrazzani FS; Hospital de Clínicas de Porto Alegre.
Jazyk:	English; Portuguese
Zdroj:	Jornal brasileiro de nefrologia : 'orgao oficial de Sociedades Brasileira e Latino-Americana de Nefrologia [J Bras Nefrol] 2017 Apr-Jun; Vol. 39 (2), pp. 181-185.
DOI:	10.5935/0101-2800.20170032
Abstrakt:	Introduction: Immunosuppression of T lymphocytes is required for preventing acute rejection after transplantation and for the treatment of chronic autoimmune and inflammatory diseases. The laboratory monitoring for this therapy is the measurement of T cells by immunophenotyping, aiming the target value of less than 20 cells per µL. Objective: To establish a cut-off point for the total number of lymphocytes in the automated blood cell count that reflects less than twenty T cells µL by immunophenotyping. Methods: We studied and evaluated 242 kidney transplant patients that had results of automated blood cell count and quantification of T cells by immunophenotyping technique. The patients were divided into two groups, depending on the T lymphocyte immunophenotyping rates established by lower and higher than 20 cells per µL. After, we evaluated the cut-off point for lymphocytes in the blood cell count with a specificity of 100% to exclude patients with high levels of T lymphocytes. Results: We found that the cut-off point of 70 lymphocytes per µL obtained by automated blood cell count showed 100% of specificity to exclude patients with T-cell counts higher than 20 cells per µL by immunophenotyping. Conclusion: The results found in this study may be helpful to monitor the immunosuppressive therapy in kidney transplant patients in places where a flow cytometer is not available, or when this equipment is not present in the full routine.
Databáze:	MEDLINE
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