Expression of Flk-1 and Cyclin D2 mRNA in the Myocardium of Rats with Doxorubicin-Induced Cardiomyopathy and after Treatment with Betulonic Acid Amide.

Autor: Mzhelskaya MM; Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia. pathol@inbox.ru., Klinnikova MG; Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia., Koldysheva EV; Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia., Lushnikova EL; Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2017 Oct; Vol. 163 (6), pp. 809-813. Date of Electronic Publication: 2017 Oct 24.
DOI: 10.1007/s10517-017-3909-5
Abstrakt: The expression of VEGFR2 (Flk-1, according to immunohistochemistry) and of cyclin D2 mRNA (according to real-time PCR) in the myocardium of rats is studied in doxorubicin-induced cardiomyopathy and in response to betulonic acid amide. Doxorubicin alone and in combination with betulonic acid amide causes after 3 days a manifest reduction of cyclin D2 mRNA expression (by 38 and 63%, respectively), while injection of betulonic acid amide alone causes a 23-fold increase of cyclin D2 mRNA expression. An increase of cyclin D2 mRNA expression has been detected in all experimental groups after 14 days of experiment, the most pronounced in response to betulonic acid amide (63 times). The expression of Flk-1 in cardiomyocytes increases significantly in response to both chemical agents starting from day 3 of experiment. These results indicate that doxorubicin and betulonic acid amide induce cytoprotective reactions in the myocardium, first at the intracellular, then at the cellular levels.
Databáze: MEDLINE
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