Analysis of Immunogenicity of Intracellular CTAR Fragments of Epstein-Barr Virus Latent Phase Protein LMP1.

Autor: Lomakin YA; M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.; Kazan (Volga Region) Federal University, Kazan, Republic of Tatarstan, Russia., Shmidt AA; M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Bobik TV; M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.; Kazan (Volga Region) Federal University, Kazan, Republic of Tatarstan, Russia., Chernov AS; Affiliated Department of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences, Pushchino, Russia., Pyrkov AY; M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Aleksandrova NM; Kazan (Volga Region) Federal University, Kazan, Republic of Tatarstan, Russia., Okunola DO; Kazan (Volga Region) Federal University, Kazan, Republic of Tatarstan, Russia., Vaskina MI; M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Ponomarenko NA; M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Telegin GB; Affiliated Department of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences, Pushchino, Russia., Dubina MV; St. Petersburg Academic University, Russian Academy of Sciences, St. Petersburg, Russia., Belogurov AA Jr; M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. belogurov@mx.ibch.ru.; Kazan (Volga Region) Federal University, Kazan, Republic of Tatarstan, Russia. belogurov@mx.ibch.ru.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2017 Oct; Vol. 163 (6), pp. 766-771. Date of Electronic Publication: 2017 Oct 24.
DOI: 10.1007/s10517-017-3899-3
Abstrakt: Intracellular fragments of latent phase protein LMP1 of Epstein-Barr virus, denoted as CTAR1/2/3, can trigger a variety of cell cascades and contribute to the transforming potential of the virus. Generation of recombinant proteins CTAR1/2/3 is expected to yield more ample data on functional and immunogenic characteristics of LMP1. We created genetic constructs for prokaryotic expression of LMP1 CTAR fragments and selected optimal conditions for their production and purification. Using a new library of LMP1 CTAR fragments, we carried out epitope mapping of a diagnostic anti-LMP1 antibody S12. Analysis of polyclonal serum antibodies from mice immunized with full-length LMP1 confirmed immunogenicity of CTAR elements comparable with that of full-length protein.
Databáze: MEDLINE
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