Human plasmacytoid dendritic cells acquire phagocytic capacity by TLR9 ligation in the presence of soluble factors produced by renal epithelial cells.

Autor:	Ruben JM; Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands., García-Romo GS; Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands., Breman E; Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands., van der Kooij S; Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands., Redeker A; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Arens R; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., van Kooten C; Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: kooten@lumc.nl.
Jazyk:	angličtina
Zdroj:	Kidney international [Kidney Int] 2018 Feb; Vol. 93 (2), pp. 355-364. Date of Electronic Publication: 2017 Oct 20.
DOI:	10.1016/j.kint.2017.08.006
Abstrakt:	Plasmacytoid dendritic cells (pDCs) are antigen presenting cells specialized in viral recognition through Toll-like receptor (TLR)7 and TLR9, and produce vast amounts of interferon alpha upon ligation of these TLRs. We had previously demonstrated a strong influx of pDCs in the tubulointerstitium of renal biopsies at the time of acute rejection. However, the role of human pDCs in mediating acute or chronic allograft rejection remains elusive. pDCs are thought to have a limited capacity to ingest apoptotic cells, critical for inducing CD4 + T cell activation via indirect antigen presentation and subsequent activation of antibody producing B cells. Here we tested whether the function of pDCs is affected by their presence within the graft. Maturation and interferon alpha production by pDCs was enhanced when cells were activated in the presence of viable HK2 renal epithelial cells. Importantly, soluble factors produced by cytomegalovirus-infected (primary) epithelial or endothelial cells enhanced pDC activation and induced their capacity to phagocytose apoptotic cells. Phagocytosis was not induced by free virus or soluble factors from non-infected cells. Activated pDCs showed an enhanced CD4 + and CD8 + T cell allostimulatory capacity as well as a potent indirect alloantigen presentation. Granulocyte Macrophage-Colony Stimulating Factor is one of the soluble factors produced by renal epithelial cells that, combined with TLR9 ligation, induced this functional capacity. Thus, pDCs present in the rejecting allograft can contribute to alloimmunity and potentially act as important orchestrators in the manifestation of acute and chronic rejection. (Copyright © 2017 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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