Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development.

Autor: Panina SB; Academy of Biology and Biotechnology, Southern Federal University, 194/1 Stachki Street, 344090, Rostov-on-Don, Russian Federation. tailana703@gmail.com., Krolevets IV; Department of Traumatology and Orthopaedics, Rehabilitation and Sport Medicine, Rostov State Medical University, 29 Nakhichevanskii St, Rostov-on-Don, 344022, Russian Federation., Milyutina NP; Academy of Biology and Biotechnology, Southern Federal University, 194/1 Stachki Street, 344090, Rostov-on-Don, Russian Federation., Sagakyants AB; Academy of Biology and Biotechnology, Southern Federal University, 194/1 Stachki Street, 344090, Rostov-on-Don, Russian Federation., Kornienko IV; Academy of Biology and Biotechnology, Southern Federal University, 194/1 Stachki Street, 344090, Rostov-on-Don, Russian Federation., Ananyan AA; Academy of Biology and Biotechnology, Southern Federal University, 194/1 Stachki Street, 344090, Rostov-on-Don, Russian Federation., Zabrodin MA; Department of Traumatology and Orthopaedics, Rehabilitation and Sport Medicine, Rostov State Medical University, 29 Nakhichevanskii St, Rostov-on-Don, 344022, Russian Federation., Plotnikov AA; Academy of Biology and Biotechnology, Southern Federal University, 194/1 Stachki Street, 344090, Rostov-on-Don, Russian Federation., Vnukov VV; Academy of Biology and Biotechnology, Southern Federal University, 194/1 Stachki Street, 344090, Rostov-on-Don, Russian Federation.
Jazyk: angličtina
Zdroj: Journal of orthopaedics and traumatology : official journal of the Italian Society of Orthopaedics and Traumatology [J Orthop Traumatol] 2017 Dec; Vol. 18 (4), pp. 349-357. Date of Electronic Publication: 2017 Oct 20.
DOI: 10.1007/s10195-017-0473-8
Abstrakt: Background: The identification of biomarkers of post-traumatic osteoarthritis (PTOA) progression is of clinical importance. The aims of this study were: (1) to assess the abilities of various soluble proinflammatory mediators in plasma to distinguish patients with knee PTOA from controls; (2) to determine the correlations between the mediators in plasma and those mediators in synovial fluid (SF); and (3) to explore the associations of the mediators with radiographic PTOA severity.
Materials and Methods: The concentrations of IL-1β, IL-6, IL-18, TNFα, and leptin were measured using ELISA. Nitric oxide was determined as nitrite/nitrate (NO x ) using the Griess reaction.
Results: We included 171 subjects (134 PTOA patients and 37 controls) and excluded patients with rheumatoid arthritis or gout. The ROC curve of plasma NO x had the highest AUC, a specificity of 100%, and a sensitivity of 84.4%. The combination of IL-6 and leptin proved to be the most discriminatory, with an AUC value of 0.933, a specificity of 96.7%, and a sensitivity of 85.7%. The levels of NO x , IL-6, IL-18, and leptin in plasma were significantly correlated with their levels in SF. Leptin levels in both plasma (p = 0.036) and SF (p = 0.041) and the synovial IL-18 level (p = 0.045) were correlated with the Kellgren-Lawrence (KL) grade. Early-stage PTOA (KL 1-2) was associated with a high concentration of IL-1β in plasma before and after (OR 6.235, 95% CI 1.362 to 28.552, p = 0.018) adjusting for age, gender, and BMI.
Conclusions: Circulating NO x level and a combination of IL-6 and leptin permitted the strongest discrimination of patients with PTOA from controls. PTOA severity was correlated with leptin levels in plasma and SF and with the synovial IL-18 level. Early PTOA was associated with the circulating level of IL-1β.
Level of Evidence: III (case-control study).
Databáze: MEDLINE
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