Molecular detection and characterization of zoonotic Anaplasma species in domestic dogs in Lusaka, Zambia.

Autor: Vlahakis PA; Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia; Department of Basic Sciences, School of Medicine, Copperbelt University, Kitwe, Zambia., Chitanga S; Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia. Electronic address: schitanga@gmail.com., Simuunza MC; Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia., Simulundu E; Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia., Qiu Y; Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia., Changula K; Department of Paraclinical studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia., Chambaro HM; Department of Veterinary Services, Ministry of Fisheries and Livestock, Lusaka, Zambia., Kajihara M; Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan., Nakao R; Unit of Risk Analysis and Management, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan., Takada A; Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia; Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan., Mweene AS; Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
Jazyk: angličtina
Zdroj: Ticks and tick-borne diseases [Ticks Tick Borne Dis] 2018 Jan; Vol. 9 (1), pp. 39-43. Date of Electronic Publication: 2017 Oct 17.
DOI: 10.1016/j.ttbdis.2017.10.010
Abstrakt: Although tick-borne pathogens, Anaplasma platys and Anaplasma phagocytophilum are recognized as zoonotic agents associated with appreciable morbidity and mortality in dogs and humans worldwide, there is limited information on these infections in many African countries, including Zambia. The purpose of this study was to detect, identify and phylogenetically characterize Anaplasma species from dogs in Chilanga District in Lusaka Province, Zambia. A total of 301 blood samples were collected from apparently healthy and semi-confined dogs. Initial screening by polymerase chain reaction with specific primers targeting the 16S rRNA gene of Anaplasma species revealed that 9% (27/301) of our samples were positive. Subsequent sequence and phylogenetic analysis of a longer fragment of the 16S rRNA and citrate synthase (gltA) genes of four positive samples showed the presence of A. platys and an Anaplasma species, which was closely related to those detected in dogs in South Africa. This is the first report on molecular identification and characterization of canine-associated zoonotic Anaplasma species in Zambia.
(Copyright © 2017 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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