Short communication: The first report of Cyberlindnera rhodanensis associated with clinical bovine mastitis.

Autor:	Dalanezi FM; Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu/SP, 18618681, Brazil., Souza da Paz G; Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu/SP, 18618681, Brazil., Joaquim SF; Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu/SP, 18618681, Brazil., Guimarães FF; Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu/SP, 18618681, Brazil., Bosco SMG; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, São Paulo State University, Botucatu/SP, 18618681, Brazil., Langoni H; Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu/SP, 18618681, Brazil. Electronic address: hlangoni@fmvz.unesp.br.
Jazyk:	angličtina
Zdroj:	Journal of dairy science [J Dairy Sci] 2018 Jan; Vol. 101 (1), pp. 581-583. Date of Electronic Publication: 2017 Oct 18.
DOI:	10.3168/jds.2017-13347
Abstrakt:	An acute case of clinical mastitis in a Holstein cow from second lactation is reported here. A milk sample from the affected quarter was cultured on 5% bovine blood agar and incubated at 37°C for 72 h. After 24 h of incubation, numerous colonies of yeast were observed: the Candida characteristic was not detected by CHROMagar Candida (Difco, Franklin Lakes, NJ). The DNA extraction of the isolate was performed, and DNA was subjected to amplification and sequencing of the D1/D2 region of the large subunit rRNA gene. The sequences were aligned using Mega 7.0 and used for searching GenBank by BLASTn (Basic Local Alignment Search Tool for nucleotides), revealing 98% of identity with Cyberlindnera rhodanensis. To date, this is the first report of this yeast associated with clinical bovine mastitis. (Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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