| Autor: |
Quasem I; Department of Biology., Luby CJ; Department of Chemistry, Tufts University, Medford, MA., Mace CR; Department of Chemistry, Tufts University, Medford, MA., Fuchs SM; Department of Biology. |
| Jazyk: |
angličtina |
| Zdroj: |
BioTechniques [Biotechniques] 2017 Oct 01; Vol. 63 (4), pp. 169-173. Date of Electronic Publication: 2017 Oct 01. |
| DOI: |
10.2144/000114596 |
| Abstrakt: |
As yeast are starved of nutrients, they enter G0, a quiescent state. Quiescent yeast (Q) cells retain viability for extended periods of time and resume growth following supplementation of missing nutrients. As such, Q cells have become a valuable model for studying longevity and self-renewal of chronologically aged cells. Traditional isolation of Q cells involves a relatively long centrifugation time through a continuous density gradient. Here, we describe a rapid and cost-effective Q-cell isolation technique that uses a single-density, one-step gradient prepared from media containing iodixanol. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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