Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing.

Autor:	Orebaugh CD; Genome Integrity and Structural Biology Laboratory, National Institute for Environmental Health Sciences, National Institute of Health (NIH), 111 TW Alexander Drive, Research Triangle Park, NC, 27709, USA., Lujan SA; Genome Integrity and Structural Biology Laboratory, National Institute for Environmental Health Sciences, National Institute of Health (NIH), 111 TW Alexander Drive, Research Triangle Park, NC, 27709, USA., Burkholder AB; Integrative Bioinformatics, National Institute for Environmental Health Sciences, National Institute of Health (NIH), Research Triangle Park, NC, USA., Clausen AR; Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden., Kunkel TA; Genome Integrity and Structural Biology Laboratory, National Institute for Environmental Health Sciences, National Institute of Health (NIH), 111 TW Alexander Drive, Research Triangle Park, NC, 27709, USA. kunkel@nih.gov.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1672, pp. 329-345.
DOI:	10.1007/978-1-4939-7306-4_23
Abstrakt:	Ribonucleotides embedded within DNA render the DNA sensitive to the formation of single-stranded breaks under alkali conditions. Here, we describe a next-generation sequencing method called hydrolytic end sequencing (HydEn-seq) to map ribonucleotides inserted into the genome of Saccharomyce cerevisiae strains deficient in ribonucleotide excision repair. We use this method to map several genomic features in wild-type and replicase variant yeast strains.
Databáze:	MEDLINE
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