Prediction of Protein Complexes in Trypanosoma brucei by Protein Correlation Profiling Mass Spectrometry and Machine Learning.
| Autor: | Crozier TWM; From the ‡Division of Biological Chemistry and Drug Discovery and.; §Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD2 1NW, UK., Tinti M; From the ‡Division of Biological Chemistry and Drug Discovery and., Larance M; §Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD2 1NW, UK., Lamond AI; §Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD2 1NW, UK m.a.j.ferguson@dundee.ac.uk a.i.lamond@dundee.ac.uk., Ferguson MAJ; From the ‡Division of Biological Chemistry and Drug Discovery and m.a.j.ferguson@dundee.ac.uk a.i.lamond@dundee.ac.uk. |
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| Jazyk: | angličtina |
| Zdroj: | Molecular & cellular proteomics : MCP [Mol Cell Proteomics] 2017 Dec; Vol. 16 (12), pp. 2254-2267. Date of Electronic Publication: 2017 Oct 17. |
| DOI: | 10.1074/mcp.O117.068122 |
| Abstrakt: | A disproportionate number of predicted proteins from the genome sequence of the protozoan parasite Trypanosoma brucei , an important human and animal pathogen, are hypothetical proteins of unknown function. This paper describes a protein correlation profiling mass spectrometry approach, using two size exclusion and one ion exchange chromatography systems, to derive sets of predicted protein complexes in this organism by hierarchical clustering and machine learning methods. These hypothesis-generating proteomic data are provided in an open access online data visualization environment (http://134.36.66.166:8083/complex_explorer). The data can be searched conveniently via a user friendly, custom graphical interface. We provide examples of both potential new subunits of known protein complexes and of novel trypanosome complexes of suggested function, contributing to improving the functional annotation of the trypanosome proteome. Data are available via ProteomeXchange with identifier PXD005968. (© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.) |
| Databáze: | MEDLINE |
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