Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice.

Autor: Gies V; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Médalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France., Bouis D; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Médalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France., Martin M; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Médalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.; UFR Médecine, Université de Strasbourg, Strasbourg, France., Pasquali JL; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Médalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.; UFR Médecine, Université de Strasbourg, Strasbourg, France., Martin T; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Médalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.; UFR Médecine, Université de Strasbourg, Strasbourg, France., Korganow AS; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Médalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.; UFR Médecine, Université de Strasbourg, Strasbourg, France., Soulas-Sprauel P; CNRS UPR 3572 'Immunopathology and Therapeutic Chemistry'/Laboratory of Excellence Médalis, Institute of Molecular and Cellular Biology (IBMC), Strasbourg, France. pauline.soulas@ibmc-cnrs.unistra.fr.; Department of Clinical Immunology and Internal Medicine, National Reference Center for Autoimmune Diseases, Hôpitaux Universitaires de Strasbourg, Strasbourg, France. pauline.soulas@ibmc-cnrs.unistra.fr.; UFR Sciences pharmaceutiques, Université de Strasbourg, Illkirch-Graffenstaden, France. pauline.soulas@ibmc-cnrs.unistra.fr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Oct 16; Vol. 7 (1), pp. 13232. Date of Electronic Publication: 2017 Oct 16.
DOI: 10.1038/s41598-017-13422-z
Abstrakt: The phenotypic characterization of self-reactive B cells producing autoantibodies is one of the challenges to get further insight in the physiopathology of autoimmune diseases. We took advantage of our previously developed flow cytometry method, using labeled nucleosomes, prominent autoantigens in systemic lupus erythematosus, to analyze the phenotype of self-reactive B cells in the anti-DNA B6.56R mouse model. We showed that splenic anti-nucleosome B cells express mostly kappa light chains and harbor a marginal zone phenotype. Moreover, these autoreactive B cells fail to acquire a germinal center phenotype and are less abundant in the transitional T3 compartment. In conclusion, the direct detection of autoreactive B cells helped determine their phenotypic characteristics and provided a more direct insight into the B cell tolerance process in B6.56R mice. This method constitutes an interesting new tool to study the mechanisms of B cell tolerance breakdown in B6.56R mice crossed with autoimmune prone models.
Databáze: MEDLINE
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