Cationic nanoemulsions as nucleic acids delivery systems.

Autor: Teixeira HF; Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Farmácia, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil. Electronic address: helder.teixeira@ufrgs.br., Bruxel F; Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Farmácia, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Pampa (UNIPAMPA), Faculdade de Farmácia, Estrada BR 472 Km7, Prédio 700, 97500970, Uruguaiana, RS, Brazil., Fraga M; Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Farmácia, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil; Centro de Terapia Gênica - Hospital de Clinicas de Porto Alegre, R. Ramiro Barcelos 2350, 90035-903, Porto Alegre, RS, Brazil; Curso de Farmácia da Universidade de Caxias do Sul (UCS), R. Francisco Getúlio Vargas 1130, 95070-560, Caxias do Sul, RS, Brazil., Schuh RS; Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Farmácia, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil; Centro de Terapia Gênica - Hospital de Clinicas de Porto Alegre, R. Ramiro Barcelos 2350, 90035-903, Porto Alegre, RS, Brazil., Zorzi GK; Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Farmácia, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil., Matte U; Programa de Pós-Graduação em Genética e Biologia Molecular da Universidade Federal do Rio Grande do Sul (UFRGS), Campus do Vale, Av. Bento Gonçalves, 9500, 91501-970, Porto Alegre, RS, Brazil; Centro de Terapia Gênica - Hospital de Clinicas de Porto Alegre, R. Ramiro Barcelos 2350, 90035-903, Porto Alegre, RS, Brazil., Fattal E; Laboratoire de Physico-chimie, Pharmacotechnie et Biopharmacie, Univ Paris-Sud, UMR 8612 CNRS, 5 Rue Jean-Baptiste Clément, 92296 Châtenay Malabry, France.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2017 Dec 20; Vol. 534 (1-2), pp. 356-367. Date of Electronic Publication: 2017 Oct 13.
DOI: 10.1016/j.ijpharm.2017.10.030
Abstrakt: Since the first clinical studies, knowledge in the field of gene therapy has advanced significantly, and these advances led to the development and subsequent approval of the first gene medicines. Although viral vectors-based products offer efficient gene expression, problems related to their safety and immune response have limited their clinical use. Thus, design and optimization of nonviral vectors is presented as a promising strategy in this scenario. Nonviral systems are nanotechnology-based products composed of polymers or lipids, which are usually biodegradable and biocompatible. Cationic liposomes are the most studied nonviral carriers and knowledge about these systems has greatly evolved, especially in understanding the role of phospholipids and cationic lipids. However, the search for efficient delivery systems aiming at gene therapy remains a challenge. In this context, cationic nanoemulsions have proved to be an interesting approach, as their ability to protect and efficiently deliver nucleic acids for diverse therapeutic applications has been demonstrated. This review focused on cationic nanoemulsions designed for gene therapy, providing an overview on their composition, physicochemical properties, and their efficacy on biological response in vitro and in vivo.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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