Tuning the Multifunctionality of Iron Oxide Nanoparticles Using Self-Assembled Mixed Lipid Layers.

Autor:	Preiss MR; Department of Chemical Engineering, University of Rhode Island , 51 Lower College Road, Kingston, Rhode Island 02881, United States., Cournoyer E; Department of Chemical Engineering, University of Rhode Island , 51 Lower College Road, Kingston, Rhode Island 02881, United States., Paquin KL; Department of Cell and Molecular Biology, University of Rhode Island , 379 CBLS, 120 Flagg Road, Kingston, Rhode Island 02881, United States., Vuono EA; Department of Cell and Molecular Biology, University of Rhode Island , 379 CBLS, 120 Flagg Road, Kingston, Rhode Island 02881, United States., Belanger K; Department of Chemical Engineering, University of Rhode Island , 51 Lower College Road, Kingston, Rhode Island 02881, United States., Walsh E; Department of Neuroscience, Department of Diagnostic Imaging, Institute for Brain Science, Institute for Molecular and Nanoscale Innovation, Associate Director for MRI Physics, Brown University , Sidney E. Frank Hall, 185 Meeting Street, Providence, Rhode Island 02912, United States., Howlett NG; Department of Cell and Molecular Biology, University of Rhode Island , 379 CBLS, 120 Flagg Road, Kingston, Rhode Island 02881, United States., Bothun GD; Department of Chemical Engineering, University of Rhode Island , 51 Lower College Road, Kingston, Rhode Island 02881, United States.
Jazyk:	angličtina
Zdroj:	Bioconjugate chemistry [Bioconjug Chem] 2017 Nov 15; Vol. 28 (11), pp. 2729-2736. Date of Electronic Publication: 2017 Oct 30.
DOI:	10.1021/acs.bioconjchem.7b00483
Abstrakt:	We present an approach to tuning the multifunctionality of iron oxide nanoparticles (IONs) using mixed self-assembled monolayers of cationic lipid and anionic polyethylene glycol (PEG) lipid. By forming stable, monodispersed lipid-coated IONs (L-IONs) through a solvent-exchange technique, we were able to demonstrate the relationship between surface charge, the magnetic transverse relaxivity (r 2 from T 2 -weighted images), and the binding capacity of small interfering ribonucleic acids (siRNAs) as a function of the cationic-to-anionic (PEG) lipid ratio. These properties were controlled by the cationic charge and the PEG conformation; relaxivity and siRNA binding could be varied in the mushroom and brush regimes but not at high brush densities. In vitro results combining cell viability, uptake, and transfection efficiency using HeLa cells suggest that the functional physicochemical and biological properties of L-IONs may be best achieved using catanionic lipid coatings near equimolar ratios of cationic to anionic PEG-lipids.
Databáze:	MEDLINE
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