Detection of Brucella abortus B19 strain DNA in seminal plasma by polymerase chain reaction in Brazil.

Autor: Junqueira Junior DG; Faculdade de Medicina Veterinária, Centro Colaborador de Defesa Agropecuária do Brasil Central, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brasil., Lima AMC; Faculdade de Medicina Veterinária, Centro Colaborador de Defesa Agropecuária do Brasil Central, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brasil., Rosinha GMS; Centro Nacional de Pesquisa em Gado de Corte, Empresa Brasileira de Pesquisa Agropecuária, Campo Grande, Mato Grosso do Sul, Brasil., Carvalho CEG; Centro Nacional de Pesquisa em Gado de Corte, Empresa Brasileira de Pesquisa Agropecuária, Campo Grande, Mato Grosso do Sul, Brasil., Oliveira CE; Centro Nacional de Pesquisa em Gado de Corte, Empresa Brasileira de Pesquisa Agropecuária, Campo Grande, Mato Grosso do Sul, Brasil., Sanches CC; Centro Nacional de Pesquisa em Gado de Corte, Empresa Brasileira de Pesquisa Agropecuária, Campo Grande, Mato Grosso do Sul, Brasil.
Jazyk: angličtina
Zdroj: Transboundary and emerging diseases [Transbound Emerg Dis] 2018 Apr; Vol. 65 (2), pp. 476-479. Date of Electronic Publication: 2017 Oct 16.
DOI: 10.1111/tbed.12727
Abstrakt: A total of 27 seminal plasma samples from cattle-breeding farms or semen centres located in Minas Gerais, Brazil, previously negative by serological and tested positive for Brucella spp. with primer specific for the amplification of the gene virb5 by polymerase chain reaction (PCR) were analysed for the detection of Brucella abortus DNA by PCR. It was found that nine samples (33.33%) contained B. abortus B19 strain DNA, two (7.40%) contained B. abortus DNA and five (18.51%) contained both DNA. The larger number of samples with B. abortus B19 strain DNA would explained by the environmental contamination by vaccinated females with persistent excretion or some illegal vaccination process. It is first reported of male bovines detected with both DNA.
(© 2017 Blackwell Verlag GmbH.)
Databáze: MEDLINE
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