Complete structure and variation of the chloroplast genome of Agropyron cristatum (L.) Gaertn.

Autor: Chen N; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan 625014, Sichuan, China., Sha LN; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Dong ZZ; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Tang C; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Wang Y; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Kang HY; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Zhang HQ; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China., Yan XB; College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China., Zhou YH; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan 625014, Sichuan, China., Fan X; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan 625014, Sichuan, China. Electronic address: fanxing9988@163.com.
Jazyk: angličtina
Zdroj: Gene [Gene] 2018 Jan 15; Vol. 640, pp. 86-96. Date of Electronic Publication: 2017 Oct 13.
DOI: 10.1016/j.gene.2017.10.009
Abstrakt: Agropyron cristatum (L.) Gaertner, a perennial grass in the tribe Triticeae (Poaceae), is a wild relative of cereal crops that is suitable for genetic improvement. In this study, we first sequenced the complete chloroplast (cp) genome of Ag. cristatum using Hiseq4000 PE150. The Ag. cristatum chloroplast genome is 135,554bp in length, has a typical quadripartite structure and contains 76 protein-coding genes, 29 tRNA genes and four rRNA genes. The cp genome of Ag. cristatum was used for comparison with other seven Triticeae species. One large variable region (800bp), which primarily contained the rpl23 (non-reciprocally translocated from IRs) and accD genes, was detected between rbcL gene and psaI gene within LSC region. The deletion of the accD and translocated rpl23 genes in Ag. cristatum indicated an independent gene-loss events or an additional divergence in Triticeae. Analyses of the dn/ds ratio and K2-P's genetic distance for 76 protein-coding genes showed that genes with evolutionary divergence might suffer from the effect of sequence regional constraints or gene functional constraints in Triticeae species. Our research will generally contribute to the knowledge of plastid genome evolution in Triticeae.
(Copyright © 2017. Published by Elsevier B.V.)
Databáze: MEDLINE
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