Serologically diagnosed acute human bocavirus 1 infection in childhood community-acquired pneumonia.

Autor: Nascimento-Carvalho AC; Bahiana School of Medicine, Bahiana Foundation for Science Development, Salvador, Brazil., Vilas-Boas AL; Department of Pediatrics, Federal University of Bahia School of Medicine, Salvador, Brazil., Fontoura MH; Department of Pediatrics, Federal University of Bahia School of Medicine, Salvador, Brazil., Xu M; Department of Virology, University of Helsinki, Helsinki, Finland., Vuorinen T; Department of Clinical Virology Turku University Hospital and Department of Virology, University of Turku, Turku, Finland., Söderlund-Venermo M; Department of Virology, University of Helsinki, Helsinki, Finland., Ruuskanen O; Department of Pediatrics, University of Turku, Turku, Finland., Nascimento-Carvalho CM; Department of Pediatrics, Federal University of Bahia School of Medicine, Salvador, Brazil.
Jazyk: angličtina
Zdroj: Pediatric pulmonology [Pediatr Pulmonol] 2018 Jan; Vol. 53 (1), pp. 88-94. Date of Electronic Publication: 2017 Oct 13.
DOI: 10.1002/ppul.23891
Abstrakt: Aim: To assess the role of human bocavirus 1 (HBoV1) as a causative agent of non-severe community-acquired pneumonia (CAP) in children.
Methods: Patients aged 2-59 months with non-severe CAP (respiratory complaints and radiographic pulmonary infiltrate/consolidation) attending a University Hospital in Salvador, Brazil were enrolled in a prospective cohort. From 820 recruited children in a clinical trial (ClinicalTrials.gov NCT01200706), nasopharyngeal aspirate (NPA), and acute and convalescent serum samples were obtained from 759 (92.6%) patients. NPAs were tested for 16 respiratory viruses by PCR. Acute HBoV1 infection was confirmed by measuring specific IgM and IgG responses in paired serum samples.
Results: Respiratory viruses were detected in 693 (91.3%; 95%CI: 89.1-93.2) CAP cases by PCR. HBoV1-DNA was detected in 159 (20.9%; 95%CI: 18.2-24.0) cases. Of these 159 PCR positive cases, acute HBoV1 infection was confirmed serologically in 38 cases (23.9%; 95%CI: 17.8-31.0). Overall, acute HBoV1 infection was confirmed in 5.0% (38/759) of non-severe CAP patients. HBoV1 was detected in 151 cases with at least one other virus making 31.7% of all multiple virus (n = 477) detections. Among all 759 cases, 216 had one respiratory virus detected, and sole HBoV1 was detected in only 8 (3.7%). Acute HBoV1 infection was serologically diagnosed in 34 (22.5%) HBoV1-DNA-positive cases with another virus, compared to 4 (50.0%) cases with sole virus detection (p = 0.09).
Conclusion: HBoV1 was detected by PCR in one fifth of the children with non-severe CAP and acute HBoV1 infection was serologically confirmed in one quarter of these cases.
(© 2017 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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