Morphological characterization of ckd in cats: Insights of fibrogenesis to be recognized.

Autor:	Morais GB; Comparative Morphology Laboratory, Universidade Estadual do Ceará, Ceará, Brazil., Viana DA; Laboratory of Veterinary Pathology and Legal Medicine Universidade Estadual do Ceará, Ceará, Brazil., Verdugo JM; Institute Cavanilles of Evolutionary Biodiversity, Universitad de Valencia, Valencia, Spain., Roselló MG; College of Veterinary Medicine, Universitad Cardenal Herrera, Spain., Porcel JO; College of Veterinary Medicine, Universitad Cardenal Herrera, Spain., Rocha DD; Laboratory of Experimental Oncology, Universidade Federal do Ceará, Ceará, Brazil., Xavier Júnior FAF; Comparative Morphology Laboratory, Universidade Estadual do Ceará, Ceará, Brazil., Barbosa KDSM; Comparative Morphology Laboratory, Universidade Estadual do Ceará, Ceará, Brazil., Silva FMO; Comparative Morphology Laboratory, Universidade Estadual do Ceará, Ceará, Brazil., Brito GAC; Laboratory of Morphology and Image Processing, Universidade Federal do Ceará, Ceará, Brazil., Sampaio CMS; Health Sciences Center, Universidade Federal do Ceará, Ceará, Brazil., Evangelista JSAM; Comparative Morphology Laboratory, Universidade Estadual do Ceará, Ceará, Brazil.
Jazyk:	angličtina
Zdroj:	Microscopy research and technique [Microsc Res Tech] 2018 Jan; Vol. 81 (1), pp. 46-57. Date of Electronic Publication: 2017 Oct 12.
DOI:	10.1002/jemt.22955
Abstrakt:	Renal fibrosis is characterized by glomerulosclerosis and tubulointerstitial fibrosis and its pathogenesis is associated with the activity of mesenchymal cells (fibroblasts), being essentially characterized by a process of excessive accumulation resulting from the deposition of extracellular matrix components. The aim of this study was to characterize the morphological presentation of chronic and fibrotic lesions in the glomerular, tubular, interstitial, and vascular compartments in feline CKD, as well as the possible participation of myofibroblasts in renal fibrotic processes in this species. Cat kidneys were collected and processed according to the conventional techniques for light microscopy, circular polarization, immunohistochemistry, and electron microscopy. Fibrotic alterations were present in all compartments analyzed. The main findings in the glomerular compartment were different degrees of glomerular sclerosis, synechia formation, Bowman's capsule calcification, in addition to glomerular basement membrane thickening and pericapsular fibrosis. The tubulointerstitial compartment had intense tubular degeneration and the immunostaining in tubular cells for mesenchymal cell markers demonstrated the possibility of mesenchymal epithelial transition and consequent involvement of myofibroblasts in the development of interstitial tubule damage. Infiltration of inflammatory cells, added to vessel thickening and fibrosis, demonstrated the severity and role of inflammation in the development and perpetuation of damage. Thus, we may conclude that fibrotic lesions play a relevant role in feline CKD and the mechanism of perpetuation of these lesions need further elucidation regarding the origin and participation of myofibroblasts and consequent mesenchymal epithelial transition in this species. (© 2017 Wiley Periodicals, Inc.)
Databáze:	MEDLINE
Externí odkaz:	Plný text