| Autor: |
Baskari S; 1 Department of Biochemistry, Osmania University, Hyderabad, India., Govatati S; 2 Department of Biochemistry, Andhra University, Visakhapatnam, India., Madhuri V; 1 Department of Biochemistry, Osmania University, Hyderabad, India., Nallabelli N; 3 Council of Scientific & Industrial Research (CSIR)-Institute of Microbial Technology, Chandigarh, India., K PM; 1 Department of Biochemistry, Osmania University, Hyderabad, India., Naik S; 1 Department of Biochemistry, Osmania University, Hyderabad, India., Poornachandar; 1 Department of Biochemistry, Osmania University, Hyderabad, India., Balka S; 1 Department of Biochemistry, Osmania University, Hyderabad, India., Tamanam RR; 2 Department of Biochemistry, Andhra University, Visakhapatnam, India., Devi VR; 1 Department of Biochemistry, Osmania University, Hyderabad, India. |
| Abstrakt: |
Progression of breast cancers often depends on hormones among which human growth hormone is prominently involved in breast cancer progression. Earlier studies have reported constitutive activation of nuclear factor-κB, a key regulator of growth hormone receptor-mediated signaling pathway in breast carcinoma, but the precise molecular mechanisms are still elusive. In this study, we investigated the effect of human growth hormone on nuclear factor-κB activation and epithelial-mesenchymal transition in breast carcinoma. Our results explored that autocrine production of human growth hormone enhances cellular proliferation by the activation of nuclear factor-κB (65 kDa) and downregulation of E-cadherin expression. Furthermore, enhanced nuclear factor-κB expression significantly increases cell proliferation and diminishes apoptosis in MCF-7 cell line. Increased expression of nuclear factor-κB significantly enhances mammary carcinoma cell migration and invasion stimulated by autocrine human growth hormone, which results in epithelial-mesenchymal transition of MCF-7 cells. In conclusion, our study revealed the influence of human growth hormone on nuclear factor-κB activity and epithelial-mesenchymal transition in mammary carcinoma. Our findings will help to understand molecular role of "growth hormone-nuclear factor-κB axis" in mammary carcinogenesis which may facilitate the discovery of suitable pathway inhibitors for disease treatment. |
