| Autor: |
Hosoda A; Department of Environmental Bioscience, Meijo University., Isomura Y; Department of Environmental Bioscience, Meijo University., Takeo S; Department of Environmental Bioscience, Meijo University., Onai T; Department of Environmental Bioscience, Meijo University., Takeuchi K; Department of Environmental Bioscience, Meijo University., Toda M; Miyoshi Oil & Fat Co., Tamura H; Department of Environmental Bioscience, Meijo University. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of oleo science [J Oleo Sci] 2017 Nov 01; Vol. 66 (11), pp. 1247-1256. Date of Electronic Publication: 2017 Oct 11. |
| DOI: |
10.5650/jos.ess17072 |
| Abstrakt: |
It is important to construct microbiological treatment systems for organic solvent-contaminated water. We developed a continuous culture supplemented with a biostimulation agent named BD-C, which is formulated from canola oil, and Xanthobacter autotrophicus strain GJ10 for an aerobic dichloromethane (DCM)-dechlorinating microorganism. The continuous culture was a chemostat constructed using a 1 L screw-capped bottle containing artificial wastewater medium with 2.0 mM DCM and 1.0% (v/v) BD-C. The expression of genes for DCM metabolism in the dechlorinating aerobe was monitored and analyzed by reverse transcription-quantitative PCR. Strain GJ10 was able to dechlorinate approximately 74% of the DCM in medium supplemented with BD-C during 12 days of incubation. The DCM dechlorination rate was calculated to be 0.11 mM/day. The ΔΔ C T method showed that expression of haloalkane dehalogenase increased 5.4 times in the presence of BD-C. Based on batch culture growth tests conducted with mineral salt medium containing three DCM concentrations (0.07, 0.20, 0.43 and 0.65 mM) with BD-C, the apparent maximum specific consumption rate (ν max ) and the saturation constant (K s ) determined for DCM degradation in this test were 19.0 nmol/h/CFU and 0.44 mM, respectively. In conclusion, BD-C enhanced the aerobic degradation of DCM by strain GJ10. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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