The effect of killer cell immunoglobulin-like receptor genotype on outcome of hematopoietic stem cell transplantation from matched sibling.

Autor: Elfishawi SM; Bone Marrow Transplantation Laboratory Unit, National Cancer Institute, Cairo University, Egypt., Mossallam GI; Bone Marrow Transplantation Laboratory Unit, National Cancer Institute, Cairo University, Egypt. Electronic address: ghada.mossallam@nci.cu.edu.eg., El-Fattah RA; Department of Medical Oncology, National Cancer Institute, Cairo University, Egypt., El-Haddad A; Department of Pediatric Oncology, National Cancer Institute, Cairo University, Egypt., Kamel AM; Bone Marrow Transplantation Laboratory Unit, National Cancer Institute, Cairo University, Egypt.
Jazyk: angličtina
Zdroj: Human immunology [Hum Immunol] 2017 Nov; Vol. 78 (11-12), pp. 684-691. Date of Electronic Publication: 2017 Oct 07.
DOI: 10.1016/j.humimm.2017.10.004
Abstrakt: The alloreactivity of natural killer (NK) cell after allogeneic hematopoietic stem cell transplantation (AHSCT) is regulated by the interaction between donor killer immunoglobulin-like receptors (KIRs) and recipient human leukocyte antigen (HLA)-class I molecules. The aim was to identify KIR genes, haplotypes and their HLA-class I ligands and to investigate their association with transplantation outcome. The study included 65 patient/donor pairs who received AHSCT from HLA-matched identical siblings. KIR genotyping was done for donors using reverse sequence specific oligonucleotide probes (rSSO) coupled with luminex technology, while HLA-C genotyping was performed in patients using rSSO strip assay. In multivariate analysis, KIR2DS4 was associated with significant reduced incidence of relapse (p = .002). A trend towards reduced incidence of relapse was also observed with more than two KIR B motifs (p = .09), whereas a significant increased relapse was associated with homozygous HLA-C2 ligand compared to combined C1/C2 and C1/C1 (p = .04). Activating KIR2DS3 was associated with rapid leukocyte engraftment (p = .02). While, KIR 2DL5 was associated with decreased CMV infection (p = .03) and better platelets engraftment (p = .05). KIR genes, haplotypes and HLA-C alleles have an impact on HSCT outcome. Better selection of donors with favorable KIR genotype can improve HLA-matched sibling HSCT outcome especially for AML patients.
(Copyright © 2017 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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