Entry Studies of New World Arenaviruses.

Autor: Martínez MG; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, USA., Forlenza MB; Laboratorio de Bioquímica y Biología del virus Junín, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)-Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Intendente Güiraldes 2160, 1428, Buenos Aires, Argentina., Candurra NA; Laboratorio de Bioquímica y Biología del virus Junín, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)-Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Intendente Güiraldes 2160, 1428, Buenos Aires, Argentina., Cordo SM; Laboratorio de Bioquímica y Biología del virus Junín, Departamento de Química Biológica (QB), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA)-Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Intendente Güiraldes 2160, 1428, Buenos Aires, Argentina. scordo@qb.fcen.uba.ar.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1604, pp. 113-133.
DOI: 10.1007/978-1-4939-6981-4_8
Abstrakt: Identification of cell moieties involved in viral binding and internalization is essential since their expression would render a cell susceptible. Further steps that allow the uncoating of the viral particle at the right subcellular localization have been intensively studied. These "entry" steps could determine cell permissiveness and often define tissue and host tropism. Therefore applying the right and, when possible, straightforward experimental approaches would shorten avenues to the complete knowledge of this first and key step of any viral life cycle. Mammarenaviruses are enveloped viruses that enter the host cell via receptor-mediated endocytosis. In this chapter we present a set of customized experimental approaches and tools that were used to describe the entry of Junín virus (JUNV), and other New World mammarenavirus members, into mammalian cells.
Databáze: MEDLINE
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