[Association of transcobalamine II gene polymorphisms and serum homocysteine, vitamin B 12 and folate levels with ulcerative colitis among Chinese patients].

Autor: Zheng S; Department of Gastroenterology, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China. wzjiangyi@yeah.net., Wu H, Ye F, Xia X, Xia S, Lin X, Wu X, Jiang L, Ding R, Jiang Y
Jazyk: čínština
Zdroj: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics [Zhonghua Yi Xue Yi Chuan Xue Za Zhi] 2017 Oct 10; Vol. 34 (5), pp. 731-736.
DOI: 10.3760/cma.j.issn.1003-9406.2017.05.025
Abstrakt: Objective: To assess the association of transcobalamine II (TCN2) gene polymorphisms and serum levels of homocysteine (Hcy), vitamin B 12 and folate with ulcerative colitis (UC) among Chinese patients.
Methods: For 397 UC patients and 574 controls, two single nucleotide polymorphisms of the TCN2 gene (rs1801198, rs9606756) were tested with an improved multiple ligase detection reaction method. Serum Hcy, vitamin B 12 and folate were measured with an enzymatic cycling assay and an chemiluminescence immunoassay, respectively.
Results: The allelic and genotypic frequencies of rs1801198 and rs9606756 did not differ significantly between the two groups (all P> 0.05). Compared with those of the control group, the frequencies of G allele and CG+GG genotype of rs1801198 were greater in patients with moderate and severe UC (both P< 0.05). The same conclusion may also be drawn for the G allele and AG genotype of rs9606756 (both P< 0.05). Compared with the controls, average Hcy level was enhanced in UC patients (P< 0.01), whereas average vitamin B 12 and folate levels were decreased in UC patients (both P< 0.01). In both groups, the average level of Hcy was lower in individuals carrying CC of (rs1801198) than in those with CG+GG (both P< 0.05). A similar conclusion was also drawn for individuals with AA of rs9606756 when compared with those carrying AG(both P< 0.05). Compared with patients with mild UC, average Hcy level was increased in those with moderate and severe UC (P< 0.01), while average vitamin B 12 and folate levels were decreased in those with moderate and severe UC (both P< 0.01). The prevalence of hyperhomocysteinemia(HHcy), vitamin B 12 deficiency and folate deficiency was greater in UC patients than in controls (all P< 0.01). In UC patients, the level of Hcy was negatively correlated with those of vitamin B 12 (P< 0.01), albumin(P< 0.01), red blood cells(P< 0.01) and platelet (P< 0.05), but positively correlated with white blood cells(P< 0.01) and Mayo score (P< 0.01). Both HHcy and folate deficiency were independent risk factors for UC (OR=4.173, OR=5.206, both P< 0.01).
Conclusion: TCN2 (rs1801198, rs9606756) variations, as well as serum levels of Hcy, vitamin B 12 and folate, are correlated with UC. Both HHcy and folate deficiency are independent risk factors for UC.
Databáze: MEDLINE
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