| Autor: |
Calin VL; Biophysics and Cellular Biotechnology Department Faculty of Medicine Carol Davila University of Medicine and Pharmacy Bucharest Romania., Mihailescu M; Physics Department Faculty of Applied Sciences Politehnica University of Bucharest Romania., Scarlat EI; Physics Department Faculty of Applied Sciences Politehnica University of Bucharest Romania., Baluta AV; Applied Electronics and Informatics Engineering Department Faculty of Electronics Telecommunications and Information Technology Politehnica University of Bucharest Romania., Calin D; Biophysics and Cellular Biotechnology Department Faculty of Medicine Carol Davila University of Medicine and Pharmacy Bucharest Romania., Kovacs E; Biophysics and Cellular Biotechnology Department Faculty of Medicine Carol Davila University of Medicine and Pharmacy Bucharest Romania., Savopol T; Biophysics and Cellular Biotechnology Department Faculty of Medicine Carol Davila University of Medicine and Pharmacy Bucharest Romania., Moisescu MG; Biophysics and Cellular Biotechnology Department Faculty of Medicine Carol Davila University of Medicine and Pharmacy Bucharest Romania. |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS open bio [FEBS Open Bio] 2017 Sep 02; Vol. 7 (10), pp. 1527-1538. Date of Electronic Publication: 2017 Sep 02 (Print Publication: 2017). |
| DOI: |
10.1002/2211-5463.12282 |
| Abstrakt: |
The cell refractive index has been proposed as a putative cancer biomarker of great potential, being correlated with cell content and morphology, cell division rate and membrane permeability. We used digital holographic microscopy to compare the refractive index and dry mass density of two B16 murine melanoma sublines of different metastatic potential. Using statistical methods, the distribution of phase shifts within the reconstructed quantitative phase images was analyzed by the method of bimodality coefficients. The observed correlation of refractive index, dry mass density and bimodality profile with the metastatic potential of the cells was validated by real time impedance-based assay and clonogenic tests. We suggest that the refractive index and bimodality analysis of quantitative phase image histograms could be developed as optical biomarkers useful in label-free detection and quantitative evaluation of cell metastatic potential. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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