Modulation of Escherichia coli UvrD Single-Stranded DNA Translocation by DNA Base Composition.

Autor:	Tomko EJ; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri., Lohman TM; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri. Electronic address: lohman@biochem.wustl.edu.
Jazyk:	angličtina
Zdroj:	Biophysical journal [Biophys J] 2017 Oct 03; Vol. 113 (7), pp. 1405-1415.
DOI:	10.1016/j.bpj.2017.08.023
Abstrakt:	Escherichia coli UvrD is an SF1A DNA helicase/translocase that functions in chromosomal DNA repair and replication of some plasmids. UvrD can also displace proteins such as RecA from DNA in its capacity as an anti-recombinase. Central to all of these activities is its ATP-driven 3'-5' single-stranded (ss) DNA translocation activity. Previous ensemble transient kinetic studies have estimated the average translocation rate of a UvrD monomer on ssDNA composed solely of deoxythymidylates. Here we show that the rate of UvrD monomer translocation along ssDNA is influenced by DNA base composition, with UvrD having the fastest rate along polypyrimidines although decreasing nearly twofold on ssDNA containing equal amounts of the four bases. Experiments with DNA containing abasic sites and polyethylene glycol spacers show that the ssDNA base also influences translocation processivity. These results indicate that changes in base composition and backbone insertions influence the translocation rates, with increased ssDNA base stacking correlated with decreased translocation rates, supporting the proposal that base-stacking interactions are involved in the translocation mechanism. (Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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