In vitro study of disinfectants on the embryonation and survival of Toxascaris leonina eggs.

Autor: El-Dakhly KM; Department of Parasitology,Faculty of Veterinary Medicine,Beni-Suef University,Beni-Suef 62511,Egypt., Aboshinaf ASM; Provincial Laboratory of Animal Health Research Institute,Dokki,Giza,Egypt., Arafa WM; Department of Parasitology,Faculty of Veterinary Medicine,Beni-Suef University,Beni-Suef 62511,Egypt., Mahrous LN; Department of Parasitology,Faculty of Veterinary Medicine,Beni-Suef University,Beni-Suef 62511,Egypt., El-Nahass E; Department of Pathology,Faculty of Veterinary Medicine,Beni-Suef University,Beni-Suef 62511,Egypt., Gharib AF; Provincial Laboratory of Animal Health Research Institute,Dokki,Giza,Egypt., Holman PJ; Department of Veterinary Pathobiology,College of Veterinary Medicine and Biomedical Sciences, Texas A&M University College Station,USA., Craig TM; Department of Veterinary Pathobiology,College of Veterinary Medicine and Biomedical Sciences, Texas A&M University College Station,USA.
Jazyk: angličtina
Zdroj: Journal of helminthology [J Helminthol] 2018 Sep; Vol. 92 (5), pp. 530-534. Date of Electronic Publication: 2017 Oct 04.
DOI: 10.1017/S0022149X17000839
Abstrakt: The effect of six available and commercial disinfectants on the embryonation and larval development of Toxascaris leonina eggs was studied. Dettol® and Virkon® both induced a 100% reduction in larval development (P ≤ 0.05). Dettol® resulted in deformed eggshells and a halt in embryonal development at 1 week post exposure. All Virkon®-treated eggs showed an early embryonic lysis 24 h post exposure. TH4+ and 70% ethanol both significantly (P ≤ 0.05) affected larval development, with 58.8 and 85.8% reduction, respectively. Neither sodium hypochlorite nor phenol significantly affected larval development (2.8 and 21.0%, respectively). Sodium hypochlorite treatment caused a visible decortication of the eggshell; however, phenol-treated embryonated Toxascaris eggs appeared more or less morphologically normal. In conclusion, the disinfectants tested induced variable degrees of decortication and suppression of larval development. Virkon®S was the most effective disinfectant against Toxascaris eggs, suggesting that it is the most advisable one to use. To the best of our knowledge, this is the first report of the use of Virkon®S as an ovicide and/or larvicide of helminths, particularly Toxascaris leonina.
Databáze: MEDLINE