Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa .

Autor:	Huberman LB; Plant and Microbial Biology Department, University of California, Berkeley, CA 94720.; Energy Biosciences Institute, University of California, Berkeley, CA 94720., Coradetti ST; Plant and Microbial Biology Department, University of California, Berkeley, CA 94720.; Energy Biosciences Institute, University of California, Berkeley, CA 94720., Glass NL; Plant and Microbial Biology Department, University of California, Berkeley, CA 94720; Lglass@berkeley.edu.; Energy Biosciences Institute, University of California, Berkeley, CA 94720.; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2017 Oct 10; Vol. 114 (41), pp. E8665-E8674. Date of Electronic Publication: 2017 Sep 25.
DOI:	10.1073/pnas.1707713114
Abstrakt:	Identifying nutrients available in the environment and utilizing them in the most efficient manner is a challenge common to all organisms. The model filamentous fungus Neurospora crassa is capable of utilizing a variety of carbohydrates, from simple sugars to the complex carbohydrates found in plant cell walls. The zinc binuclear cluster transcription factor CLR-1 is necessary for utilization of cellulose, a major, recalcitrant component of the plant cell wall; however, expression of clr-1 in the absence of an inducer is not sufficient to induce cellulase gene expression. We performed a screen for unidentified actors in the cellulose-response pathway and identified a gene encoding a hypothetical protein ( clr-3 ) that is required for repression of CLR-1 activity in the absence of an inducer. Using clr-3 mutants, we implicated the hyperosmotic-response pathway in the tunable regulation of glycosyl hydrolase production in response to changes in osmolarity. The role of the hyperosmotic-response pathway in nutrient sensing may indicate that cells use osmolarity as a proxy for the presence of free sugar in their environment. These signaling pathways form a nutrient-sensing network that allows N crassa cells to tightly regulate gene expression in response to environmental conditions. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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