3'-Deoxy-3'-[ 18 F]Fluorothymidine Uptake Is Related to Thymidine Phosphorylase Expression in Various Experimental Tumor Models.

Autor: Schelhaas S; European Institute for Molecular Imaging (EIMI), Westfälische Wilhelms-Universität (WWU) Münster, Waldeyerstr. 15, 48149, Münster, Germany., Heinzmann K; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.; Comprehensive Cancer Imaging Centre, Imperial College London, London, UK., Honess DJ; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK., Smith DM; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK., Keen H; PHB Imaging Group, AstraZeneca, Alderley Park, Macclesfield, UK., Heskamp S; Department of Radiology and Nuclear Medicine, Radboud University Medical Centre, Nijmegen, The Netherlands., Witney TH; Comprehensive Cancer Imaging Centre, Imperial College London, London, UK.; UCL Centre for Advanced Biomedical Imaging, University College London, London, UK., Besret L; Sanofi Oncology, Vitry-sur-Seine, France., Doblas S; LBI, UMR1149-CRI, INSERM, Paris, France., Griffiths JR; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK., Aboagye EO; Comprehensive Cancer Imaging Centre, Imperial College London, London, UK., Jacobs AH; European Institute for Molecular Imaging (EIMI), Westfälische Wilhelms-Universität (WWU) Münster, Waldeyerstr. 15, 48149, Münster, Germany. ahjacobs@uni-muenster.de.; Department of Geriatric Medicine, Johanniter Hospital, Bonn, Germany. ahjacobs@uni-muenster.de.
Jazyk: angličtina
Zdroj: Molecular imaging and biology [Mol Imaging Biol] 2018 Apr; Vol. 20 (2), pp. 194-199.
DOI: 10.1007/s11307-017-1125-3
Abstrakt: Purpose: We recently reported that high thymidine phosphorylase (TP) expression is accompanied by low tumor thymidine concentration and high 3'-deoxy-3'-[ 18 F]fluorothymidine ([ 18 F]FLT) uptake in four untreated lung cancer xenografts. Here, we investigated whether this relationship also holds true for a broader range of tumor models.
Procedures: Lysates from n = 15 different tumor models originating from n = 6 institutions were tested for TP and thymidylate synthase (TS) expression using western blots. Results were correlated to [ 18 F]FLT accumulation in the tumors as determined by positron emission tomography (PET) measurements in the different institutions and to previously published thymidine concentrations.
Results: Expression of TP correlated positively with [ 18 F]FLT SUV max (ρ = 0.549, P < 0.05). Furthermore, tumors with high TP levels possessed lower levels of thymidine (ρ = - 0.939, P < 0.001).
Conclusions: In a broad range of tumors, [ 18 F]FLT uptake as measured by PET is substantially influenced by TP expression and tumor thymidine concentrations. These data strengthen the role of TP as factor confounding [ 18 F]FLT uptake.
Databáze: MEDLINE
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