Ashwagandha ( Withania somnifera) supercritical CO 2 extract derived withanolides mitigates Bisphenol A induced mitochondrial toxicity in HepG2 cells.

Autor: Vidyashankar S; In Vitro Biology, Research and Development, The Himalaya Drug Company, Makali, Bangalore 562 162, India., Thiyagarajan OS; In Vitro Biology, Research and Development, The Himalaya Drug Company, Makali, Bangalore 562 162, India., Varma RS; In Vitro Biology, Research and Development, The Himalaya Drug Company, Makali, Bangalore 562 162, India., Kumar LMS; Phytochemistry, Research and Development, The Himalaya Drug Company, Makali, Bangalore 562 162, India., Babu UV; Phytochemistry, Research and Development, The Himalaya Drug Company, Makali, Bangalore 562 162, India., Patki PS; Medical Services and Clinical Trials, Research and Development, The Himalaya Drug Company, Makali, Bangalore 562 162, India.
Jazyk: angličtina
Zdroj: Toxicology reports [Toxicol Rep] 2014 Jul 02; Vol. 1, pp. 1004-1012. Date of Electronic Publication: 2014 Jul 02 (Print Publication: 2014).
DOI: 10.1016/j.toxrep.2014.06.008
Abstrakt: Bisphenol A (BPA) safety aspects on human health are debated extensively for long time. In the present study, we have studied the toxicity induced by BPA at no observed adverse effect level (NOAEL) using HepG2 cells. We report that BPA at 100 nM induced cytotoxicity to HepG2 cells as determined by MTT assay at 0-72 h. The toxicity was result of reduced oxygen consumption and reduced mitochondrial membrane potential associated with decreased ATP production. The BPA treatment resulted in increase of malondialdehyde (MDA) content with decreased glutathione and other antioxidant enzymes. BPA derived toxicity is a concern to human health and alternative non-toxic natural products/derivatives or adjuvants that serve as antidote will be relevant. In this context, Ashwagandha ( Withania somnifera) a widely used herb to treat arthritis, rheumatism and to improve longevity for time immemorial is investigated for its antidote effect. Ashwagandha supercritical CO 2 extract derived Withanolides (ADW) at 100 μg/ml protect HepG2 cells from BPA induced toxicity by suppressing mitochondrial damage and increased ATP production. Further, cellular MDA content was significantly suppressed with increased non-enzymic and antioxidant enzyme activities. These findings derived from the present study suggest the beneficial effect of ADW in mitigating BPA induced mitochondrial toxicity in HepG2 cells.
Databáze: MEDLINE
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