Low viral load of Merkel cell polyomavirus in Iranian patients with head and neck squamous cell carcinoma: Is it clinically important?

Autor: Mohebbi E; Department of Biology, Science, and Research Branch, Islamic Azad University, Tehran, Iran., Noormohamadi Z; Department of Biology, Science, and Research Branch, Islamic Azad University, Tehran, Iran., Sadeghi-Rad H; Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Sadeghi F; Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran., Yahyapour Y; Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran., Vaziri F; Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran., Rahimi A; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran., Rahimi Jamnani F; Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran., Mehrabi S; Department of Medical Genetics, Tehran University of Medical Sciences, Imam Khomeini Hospital, Tehran, Iran., Siadat SD; Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran., Noori-Daloii MR; Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran., Fateh A; Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2018 Feb; Vol. 90 (2), pp. 344-350. Date of Electronic Publication: 2017 Oct 20.
DOI: 10.1002/jmv.24953
Abstrakt: Recent studies show that the human Merkel cell polyomavirus (MCPyV) may be involved in causing cancer. The objective of this study was to assess the impact of MCPyV on the development of head and neck squamous cell carcinoma (HNSCC). In total, 50 paraffin-embedded HNSCC biopsy samples and 50 adjacent non-cancerous samples were evaluated for the presence of MCPyV DNA and RNA. Among patients, the five most frequent histopathologic sites were the tongue (22.0%), lip (16.0%), submandibular (14.0%), cheek (14.0%), and throat (14.0%). MCPyV DNA was positive in eight (16.0%) samples. The median MCPyV LT-Ag copy number in the eight positive samples and in one non-cancerous sample was 4.8 × 10 -3 and 2.6 × 10 -5  copies/cell, respectively. Quantification of MCPyV LT-Ag revealed increased expression in stage III (5.6 × 10 -3  copies/cell) than in the other stages. The MCPyV DNA load in different stages of HNSCC was also statistically significant (P = 0.027). The viral load was low, suggesting that only a fraction of cancerous cells is infected. This result provides evidence confirming the presence of MCPyV in a subset of Iranian patients with HNSCCs, but further studies needed to confirm our findings.
(© 2017 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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