Identification of a 48 kDa form of unconventional myosin 1c in blood serum of patients with autoimmune diseases.
| Autor: | Myronovkij S; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanova Street 14/16, 79005 Lviv, Ukraine., Negrych N; Danylo Halytsky Lviv National Medical University, Lviv, Ukraine., Nehrych T; Danylo Halytsky Lviv National Medical University, Lviv, Ukraine., Redowicz MJ; Nencki Institute of Experimental Biology, Warsaw, Poland., Souchelnytskyi S; College of Medicine, Qatar University, Doha, Qatar.; Oranta CancerDiagnostics AB, Uppsala, Sweden.; Odesa State Medical University, Odesa, Ukraine.; Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland., Stoika R; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanova Street 14/16, 79005 Lviv, Ukraine., Kit Y; Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanova Street 14/16, 79005 Lviv, Ukraine. |
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| Jazyk: | angličtina |
| Zdroj: | Biochemistry and biophysics reports [Biochem Biophys Rep] 2015 Dec 03; Vol. 5, pp. 175-179. Date of Electronic Publication: 2015 Dec 03 (Print Publication: 2016). |
| DOI: | 10.1016/j.bbrep.2015.12.001 |
| Abstrakt: | We searched for protein markers present in blood serum of multiple sclerosis (MS), rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients in comparison to healthy human individuals. We used precipitation/extraction methods and MALDI TOF/TOF mass spectrometry, and identified a protein with Mr ~46 kDa as a fragment of human unconventional myosin IC isoform b (Myo1C). Western blotting with specific anti-human Myo1C antibodies confirmed the identity. Screening of blood serum samples from different autoimmune patients for the presence of Myo1c revealed its high level in MS and RA patients, relatively low level in SLE patients, and undetected in healthy donors. These data are suggesting that the level of p46 Myo1C in blood serum is a potential marker for testing of autoimmune diseases. |
| Databáze: | MEDLINE |
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