Characterisation of complete high- and low-risk human papillomavirus genomes isolated from cervical specimens in southern Brazil.

Autor: Oliveira GR; Universidade Federal do Rio Grande, Escola de Medicina, Laboratório de Biologia Molecular, Rio Grande, RS, Brasil.; Instituto Nacional de Câncer, Programa de Oncovirologia, Rio de Janeiro, RJ, Brasil., Siqueira JD; Instituto Nacional de Câncer, Programa de Oncovirologia, Rio de Janeiro, RJ, Brasil., Finger-Jardim F; Universidade Federal do Rio Grande, Escola de Medicina, Laboratório de Biologia Molecular, Rio Grande, RS, Brasil., Vieira VC; Instituto Nacional de Câncer, Programa de Oncovirologia, Rio de Janeiro, RJ, Brasil., Silva RL; Universidade Federal do Rio Grande, Escola de Medicina, Rio Grande, RS, Brasil., Gonçalves CV; Universidade Federal do Rio Grande, Escola de Medicina, Centro de Obstetrícia e Ginecologia, Rio Grande, RS, Brasil., Soares EA; Instituto Nacional de Câncer, Programa de Oncovirologia, Rio de Janeiro, RJ, Brasil., Martinez AMB; Universidade Federal do Rio Grande, Escola de Medicina, Laboratório de Biologia Molecular, Rio Grande, RS, Brasil., Soares MA; Instituto Nacional de Câncer, Programa de Oncovirologia, Rio de Janeiro, RJ, Brasil.
Jazyk: angličtina
Zdroj: Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2017 Oct; Vol. 112 (10), pp. 728-731.
DOI: 10.1590/0074-02760170121
Abstrakt: The classification of human papillomavirus (HPV) intratypic lineages by complete genome sequencing is a determinant in understanding biological differences in association with this disease. In this work, we have characterised complete HPV genomes from southern Brazil. Fifteen cervicovaginal Pap smear negative samples previously categorised as HPV-positive were sequenced using ultradeep sequencing, and 18 complete genomes from 13 different HPV types were assembled. Phylogenetic and genetic distance analyses were performed to classify the HPV genomes into lineages and sublineages. This is the first report describing the distribution of HPV intratype lineages of high and low oncogenic risk in asymptomatic women from southern Brazil.
Databáze: MEDLINE
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