| Autor: |
Sazonova MA; Russian Cardiology Research and Production Complex, Moscow 121552, 15a, 3rd Cherepkovskaya street, Moscow 121552, Russia.; Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow 125315, 8, Baltiyskaya st., Moscow 125315, Russia., Sinyov VV; Russian Cardiology Research and Production Complex, Moscow 121552, 15a, 3rd Cherepkovskaya street, Moscow 121552, Russia., Ryzhkova AI; Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow 125315, 8, Baltiyskaya st., Moscow 125315, Russia.; K.I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology-MVA, 23, Skryabina st., Moscow 109472, Russia., Galitsyna EV; Department of Genetics, Southern Federal University, 105/42, B. Sadovaya st., Rostov-on-Don, 344006, Russia., Khasanova ZB; Russian Cardiology Research and Production Complex, Moscow 121552, 15a, 3rd Cherepkovskaya street, Moscow 121552, Russia., Postnov AY; Russian Cardiology Research and Production Complex, Moscow 121552, 15a, 3rd Cherepkovskaya street, Moscow 121552, Russia., Yarygina EI; K.I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology-MVA, 23, Skryabina st., Moscow 109472, Russia., Orekhov AN; Russian Cardiology Research and Production Complex, Moscow 121552, 15a, 3rd Cherepkovskaya street, Moscow 121552, Russia.; Institute for Atherosclerosis Research, 121609, Skolkovo Innovative Centre, Moscow Region, Skolkovo, Novaya st., Moscow, Russia., Sobenin IA; Russian Cardiology Research and Production Complex, Moscow 121552, 15a, 3rd Cherepkovskaya street, Moscow 121552, Russia.; Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow 125315, 8, Baltiyskaya st., Moscow 125315, Russia. |
| Abstrakt: |
Mutations of mtDNA, due to their higher frequency of occurrence compared to nuclear DNA mutations, are the most promising biomarkers for assessing predisposition of the occurrence and development of atherogenesis. The aim of the present article was an analysis of correlation of several mitochondrial genome mutations with carotid atherosclerosis. Leukocytes from blood of study participants from Moscow polyclinics were used as research material. The sample size was 700 people. The sample members were diagnosed with "atherosclerosis" on the basis of ultrasonographic examination and biochemical and molecular cell tests. DNA was isolated from blood leukocyte samples of the study participants. PCR fragments of DNA, containing the region of 11 investigated mutations, were pyrosequenced. The heteroplasmy level of these mutations was detected. Statistical analysis of the obtained results was performed using the software package SPSS 22.0. According to the obtained results, an association of mutations m.652delG, m.3336C>T, m.12315G>A, m.14459G>A m.15059G>A with carotid atherosclerosis was found. These mutations can be biomarkers for assessing predisposition to this disease. Additionally, two single nucleotide substitutions (m.13513G>A and m.14846G>A), negatively correlating with atherosclerotic lesions, were detected. These mutations may be potential candidates for gene therapy of atherosclerosis and its risk factors. |
Plný text