Lipid and protein fingerprinting for Fusarium oxysporum f. sp. cubense strain-level classification.

Autor: Rocha DFO; ThoMSon Mass Spectrometry Laboratory, University of Campinas-UNICAMP, Josué Castro Street, University City, Campinas, São Paulo, 13083-872, Brazil. drocha@iqm.unicamp.br., Cunha CMS; Flora Biotecnologia Ltda, Rua Silvio Ernesto da Silva 100, Itajaí, Santa Catarina, 88307-751, Brazil., Belaz KRA; ThoMSon Mass Spectrometry Laboratory, University of Campinas-UNICAMP, Josué Castro Street, University City, Campinas, São Paulo, 13083-872, Brazil., Dos Santos FN; ThoMSon Mass Spectrometry Laboratory, University of Campinas-UNICAMP, Josué Castro Street, University City, Campinas, São Paulo, 13083-872, Brazil., Hinz RH; Empresa Sitio Barreiras Fruticultura Ltda, Missão Velha, Ceará, 63200, Brazil., Pereira A; Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina-EPAGRI, Rodovia Antônio Heil, 6.800, Itajaí, Santa Catarina, 88112-318, Brazil., Wicket E; Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina-EPAGRI, Rodovia Antônio Heil, 6.800, Itajaí, Santa Catarina, 88112-318, Brazil., Andrade LM; Polytechnic School of the University of São Paulo, Av. Prof. Luciano Gualberto, 380, São Paulo, São Paulo, 05508-010, Brazil., Nascimento CAO; Polytechnic School of the University of São Paulo, Av. Prof. Luciano Gualberto, 380, São Paulo, São Paulo, 05508-010, Brazil., Visconti A; Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina-EPAGRI, Rodovia Antônio Heil, 6.800, Itajaí, Santa Catarina, 88112-318, Brazil., Eberlin MN; ThoMSon Mass Spectrometry Laboratory, University of Campinas-UNICAMP, Josué Castro Street, University City, Campinas, São Paulo, 13083-872, Brazil.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2017 Nov; Vol. 409 (29), pp. 6803-6812. Date of Electronic Publication: 2017 Sep 25.
DOI: 10.1007/s00216-017-0638-6
Abstrakt: Banana is one of the most popular fruits in the world but has been substantially impaired by Panama disease in the last years. Fusarium oxysporum f. sp. cubense (Foc) is the causal agent and colonizes banana cultivars from many subgroups with different aggressiveness levels, often leading to plant death while compromising new crops in infested areas. This study has evaluated the ability of MALDI-MS protein and lipid fingerprinting to provide intraspecies classification of Foc isolates and to screen biomolecules related to host-pathogen relationship. The MS data, when inspected via partial least square discriminant analysis (PLS-DA), distinguished the isolates by aggressiveness as well as by specific location and host. Although both lipids and proteins show discriminating tendencies, these differences were more clearly perceived via the protein profiles. Considering that Cavendish cultivar is the more resistant option to endure Foc presence in the field, the lipids and proteins related to this subgroup might have an important role in pathogen adaptation. This study reports a new application of MALDI-MS for the analysis of a banana pathogen with intraspecies classification ability. Graphical abstract MALDI-MS classified Foc isolates by aggressiveness level on banana revealing the additional influence of location and host cultivar on the expression of lipids and proteins.
Databáze: MEDLINE
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