Validation of a reversed-phase high-performance liquid chromatographic method for the determination of free amino acids in rice using l-theanine as the internal standard.

Autor: Liyanaarachchi GVV; Industrial Technology Institute, 363, Bauddhaloka Mawatha, Colombo 7, Sri Lanka. Electronic address: vimarshi@iti.lk., Mahanama KRR; University of Colombo, Colombo 3, Sri Lanka. Electronic address: maha@chem.cmb.ac.lk., Somasiri HPPS; Industrial Technology Institute, 363, Bauddhaloka Mawatha, Colombo 7, Sri Lanka. Electronic address: hpps@iti.lk., Punyasiri PAN; Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 3, Sri Lanka. Electronic address: pnimal@hotmail.com.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2018 Feb 01; Vol. 240, pp. 196-203. Date of Electronic Publication: 2017 Jul 14.
DOI: 10.1016/j.foodchem.2017.07.038
Abstrakt: The study presents the validation results of the method carried out for analysis of free amino acids (FAAs) in rice using l-theanine as the internal standard (IS) with o-phthalaldehyde (OPA) reagent using high-performance liquid chromatography-fluorescence detection. The detection and quantification limits of the method were in the range 2-16μmol/kg and 3-19μmol/kg respectively. The method had a wide working range from 25 to 600μmol/kg for each individual amino acid, and good linearity with regression coefficients greater than 0.999. Precision measured in terms of repeatability and reproducibility, expressed as percentage relative standard deviation (% RSD) was below 9% for all the amino acids analyzed. The recoveries obtained after fortification at three concentration levels were in the range 75-105%. In comparison to l-norvaline, findings revealed that l-theanine is suitable as an IS and the validated method can be used for FAA determination in rice.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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