Manipulating Cellular Activities Using an Ultrasound-Chemical Hybrid Tool.

Autor:	Fan CH, Huang YS, Huang WE, Lee AA; Department of Chemistry, National Taiwan University , Taipei, 10617, Taiwan., Ho SY; Department of Chemistry, National Taiwan University , Taipei, 10617, Taiwan., Kao YL, Wang CL, Lian YL, Ueno T; Graduate School of Pharmaceutical Sciences, University of Tokyo , Tokyo, 113-8654, Japan., Andrew Wang TS; Department of Chemistry, National Taiwan University , Taipei, 10617, Taiwan., Yeh CK, Lin YC
Jazyk:	angličtina
Zdroj:	ACS synthetic biology [ACS Synth Biol] 2017 Nov 17; Vol. 6 (11), pp. 2021-2027. Date of Electronic Publication: 2017 Oct 12.
DOI:	10.1021/acssynbio.7b00162
Abstrakt:	We developed an ultrasound-chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location without inducing noticeable toxicity. With this system, we demonstrated the successful modulation of phospholipid metabolism triggered by a short pulse of ultrasound exposure. Our technique offers a powerful and versatile tool for using ultrasound to spatiotemporally manipulate the cellular physiology in living cells.
Databáze:	MEDLINE
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