Cytotoxicity, genotoxicity and gene expression changes elicited by exposure of human hepatic cells to Ginkgo biloba leaf extract.

Autor: Grollino MG; Laboratory of Biosafety and Risk Assessment, ENEA CR Casaccia, Via Anguillarese 301, 00123 Rome, Italy. Electronic address: maria.grollino@enea.it., Raschellà G; Laboratory of Biosafety and Risk Assessment, ENEA CR Casaccia, Via Anguillarese 301, 00123 Rome, Italy., Cordelli E; Laboratory of Biosafety and Risk Assessment, ENEA CR Casaccia, Via Anguillarese 301, 00123 Rome, Italy., Villani P; Laboratory of Biosafety and Risk Assessment, ENEA CR Casaccia, Via Anguillarese 301, 00123 Rome, Italy., Pieraccioli M; Department of Experimental Medicine and Surgery, University of Rome 'Tor Vergata', Via Montpellier 1, 00133 Rome, Italy., Paximadas I; Unit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Pisana, Via di Val Cannuta 247, 00166 Rome, Italy., Malandrino S; Indena S.p.A., Viale Ortles 12, 20139 Milan, Italy., Bonassi S; Unit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Pisana, Via di Val Cannuta 247, 00166 Rome, Italy; Department of Human Sciences and Quality of Life Promotion, San Raffaele University, Via di Val Cannuta 247, 00166 Rome, Italy., Pacchierotti F; Laboratory of Biosafety and Risk Assessment, ENEA CR Casaccia, Via Anguillarese 301, 00123 Rome, Italy.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2017 Nov; Vol. 109 (Pt 1), pp. 486-496. Date of Electronic Publication: 2017 Sep 22.
DOI: 10.1016/j.fct.2017.09.042
Abstrakt: The use of Ginkgo biloba leaf extract as nutraceutical is becoming increasingly common. As a consequence, the definition of a reliable toxicological profile is a priority for its safe utilization. Recently, contrasting data have been reported on the carcinogenic potential of Ginkgo biloba extract in rodent liver. We measured viability, Reactive Oxygen Species (ROS), apoptosis, colony-forming efficiency, genotoxicity by comet assay, and gene expression changes associated with hepato-carcinogenicity in human cells of hepatic origin (HepG2 and THLE-2) treated with different concentrations (0.0005-1.2 mg/mL) of Ginkgoselect ® Plus. Our analyses highlighted a decrease of cell viability, not due to apoptosis, after treatment with high doses of the extract, which was likely due to ROS generation by a chemical reaction between extract polyphenols and some components of the culture medium. Comet assay did not detect genotoxic effect at any extract concentration. Finally, the array analysis detected a slight decrease in the expression of only one gene (IGFBP3) in Ginkgo-treated THLE-2 cells as opposed to changes in 28 genes in Aflatoxin B1 treated-cells. In conclusion, our results did not detect any significant genotoxic or biologically relevant cytotoxic effects and gross changes in gene expression using the Ginkgo extract in the hepatic cells tested.
(Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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