Purification, physicochemical and thermodynamic studies of antifungal chitinase with production of bioactive chitosan-oligosaccharide from newly isolated Aspergillus griseoaurantiacus KX010988.
Autor: | Shehata AN; Biochemistry Department, National Research Centre, Dokki, Giza, Egypt. Electronic address: futtur@yahoo.com., Abd El Aty AA; Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza, Egypt., Darwish DA; Molecular Biology Department, National Research Centre, Dokki, Giza, Egypt., Abdel Wahab WA; Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza, Egypt., Mostafa FA; Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza, Egypt. |
---|---|
Jazyk: | angličtina |
Zdroj: | International journal of biological macromolecules [Int J Biol Macromol] 2018 Feb; Vol. 107 (Pt A), pp. 990-999. Date of Electronic Publication: 2017 Sep 20. |
DOI: | 10.1016/j.ijbiomac.2017.09.071 |
Abstrakt: | In our search for chitinase and chitosanase producer from unconventional sources, the marine-derived fungus Aspergillus griseoaurantiacus KX010988 was obviously the best producer of the highest chitinase and chitosanase activities by solid state fermentation of potato shells. Chitinase was purified in three steps involving ammonium sulphate precipitation, DEAE-cellulose ion-exchange chromatography and Sephacryl S-300 gel chromatography. 12.55 fold increase in purity with a recovery of 17.6 was obtained. The molecular mass of the purified chitinase was found to be 130kDa. It was optimally active at pH 4.5 and 40°C. K (Copyright © 2017 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
Externí odkaz: |