The Transcriptome of Estrogen-Independent Mammary Growth in Female Mice Reveals That Not All Mammary Glands Are Created Equally.

Autor:	Berryhill GE; Department of Animal Science, University of California Davis, Davis, California 95616-8521., Lemay DG; UC Davis Genome Center, University of California Davis, Davis, California 95616-8521.; US Department of Agriculture, Agricultural Research Services, Western Human Nutrition Research Center, Davis, California 95616., Trott JF; Department of Animal Science, University of California Davis, Davis, California 95616-8521., Aimo L; Department of Animal Science, University of California Davis, Davis, California 95616-8521., Lock AL; Department of Animal Science, Michigan State University, East Lansing, Michigan 48824-1225., Hovey RC; Department of Animal Science, University of California Davis, Davis, California 95616-8521.
Jazyk:	angličtina
Zdroj:	Endocrinology [Endocrinology] 2017 Oct 01; Vol. 158 (10), pp. 3126-3139.
DOI:	10.1210/en.2017-00395
Abstrakt:	Allometric growth of ducts in the mammary glands (MGs) is widely held to be estrogen dependent. We previously discovered that the dietary fatty acid trans-10, cis-12 conjugated linoleic acid (CLA) stimulates estrogen-independent allometric growth and terminal end bud formation in ovariectomized mice. Given the similar phenotype induced by estrogen and CLA, we investigated the shared and/or divergent mechanisms underlying these changes. We confirmed MG growth induced by CLA is temporally distinct from that elicited by estrogen. We then used RNA sequencing to compare the transcriptome of the MG during similar proliferative and morphological states. Both estrogen and CLA affected the genes involved in proliferation. The transcriptome for estrogen-treated mice included canonical estrogen-induced genes, including Pgr, Areg, and Foxa1. In contrast, their expression was unchanged by CLA. However, CLA, but not estrogen, altered expression of a unique set of inflammation-associated genes, consistent with stromal changes. This CLA-altered signature included increased expression of epidermal growth factor receptor (EGFR) pathway components, consistent with the demonstration that CLA-induced MG growth is EGFR dependent. Our findings highlight a unique role for diet-induced inflammation that underlies estrogen-independent MG development. (Copyright © 2017 Endocrine Society.)
Databáze:	MEDLINE
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