Theoretical and experimental study of the antifreeze protein AFP752, trehalose and dimethyl sulfoxide cryoprotection mechanism: correlation with cryopreserved cell viability.

Autor:	Kratochvílová I; Institute of Physics, Academy of Sciences of the Czech Republic, v.v.i., Na Slovance 2, CZ-182 21, Prague 8, Czech Republic., Golan M; Institute of Physics, Academy of Sciences of the Czech Republic, v.v.i., Na Slovance 2, CZ-182 21, Prague 8, Czech Republic.; Faculty of Mathematics and Physics, Charles University in Prague, Ke Karlovu 5, CZ-121 16 Prague 2, Czech Republic., Pomeisl K; Institute of Physics, Academy of Sciences of the Czech Republic, v.v.i., Na Slovance 2, CZ-182 21, Prague 8, Czech Republic., Richter J; Institute of Physics, Academy of Sciences of the Czech Republic, v.v.i., Na Slovance 2, CZ-182 21, Prague 8, Czech Republic., Sedláková S; Institute of Physics, Academy of Sciences of the Czech Republic, v.v.i., Na Slovance 2, CZ-182 21, Prague 8, Czech Republic., Šebera J; Institute of Physics, Academy of Sciences of the Czech Republic, v.v.i., Na Slovance 2, CZ-182 21, Prague 8, Czech Republic., Mičová J; Institute of Physics, Academy of Sciences of the Czech Republic, v.v.i., Na Slovance 2, CZ-182 21, Prague 8, Czech Republic.; Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9,845 38 Bratislava 4, Slovak Republic., Falk M; Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 65 Brno, Czech Republic., Falková I; Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Kralovopolska 135, CZ-612 65 Brno, Czech Republic., Řeha D; Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Zámek 136, CZ-373 33 Nové Hrady, Czech Republic.; Faculty of Sciences, University of South Bohemia in Ceske Budejovice, Zamek 136, 373 33 Nove Hrady, Czech Republic., Elliott KW; Deparment of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, 46 College Road, Durham, NH, 03824, USA., Varga K; Deparment of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, 46 College Road, Durham, NH, 03824, USA., Follett SE; Department of Chemistry, University of Wyoming, 1000 E. University Ave, Laramie, WY, 82071, USA., Šimek D; Institute of Physics, Academy of Sciences of the Czech Republic, v.v.i., Na Slovance 2, CZ-182 21, Prague 8, Czech Republic.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2017; Vol. 7 (1), pp. 352-360. Date of Electronic Publication: 2016 Dec 23.
DOI:	10.1039/C6RA25095E
Abstrakt:	In this work the physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab-initio modelling (molecular dynamics, DFT), Raman spectroscopy, Differential Scanning Calorimetry and X-Ray Diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing. DMSO and AFP had different impacts on their solution's freezing process but in both cases the ice crystallinity size was considerably reduced. DMSO and AFP treatment in different ways improved the viability of frozen/thawed cells.
Databáze:	MEDLINE
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