Concurrent administration effect of antibiotic and anti-inflammatory drugs on the immunotoxicity of bacterial endotoxins.

Autor: El Amir AM; Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt., Tanious DG; Department of Pharmacology, National Organization of Drug Control and Research (NODCAR), Giza, Egypt. Electronic address: dollylady@outlook.com., Mansour HA; Department of Pharmacology, National Organization of Drug Control and Research (NODCAR), Giza, Egypt.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2017 Nov; Vol. 52, pp. 176-184. Date of Electronic Publication: 2017 Sep 18.
DOI: 10.1016/j.intimp.2017.09.006
Abstrakt: Pseudomonas aeruginosa (P. aeruginosa) is a gram-negative bacterium that causes a variety of diseases in compromised hosts. Bacterial endotoxins such as lipopolysaccharide (LPS) are the major outer surface membrane components that are present in almost all gram-negative bacteria and act as extremely strong stimulators of innate immunity and inflammation of the airway. This study was undertaken to determine the effect of combined administration of Gentamicin (GENT) as an antibiotic and Dexamethasone (DEXA) as an anti-inflammatory drug on some immunological and histological parameters. After determination of LD 50 of P. aeruginosa, mice groups were injected with DEXA, GENT and lipopolysaccharide alone or in combination. Lipopolysaccharide single injection caused a significant increase of total leukocyte count, lymphocytes, neutrophils and levels of IgM and IgG. DEXA induced an increase of neutrophilia and lymphopenia. Immunological examination demonstrated that combined treatment has a significant effect of decreasing lymphocytes and IgG levels than single treatment does. Histological examination demonstrated that the inflammation of thymus, spleen, lymph node and liver decreases in mice that received combined treatment than those that received individual treatment. Concurrent administration of DEXA and GENT has a great effect on protecting organs against damage in case of endotoxemia.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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