Role of induced glutathione-S-transferase from Helicoverpa armigera (Lepidoptera: Noctuidae) HaGST-8 in detoxification of pesticides.

Autor: Labade CP; Institute of Bioinformatics and Biotechnology (IBB), Savitribai Phule Pune University, (SPPU), Ganeshkhind Road, Pune 411007, Maharashtra, India., Jadhav AR; Institute of Bioinformatics and Biotechnology (IBB), Savitribai Phule Pune University, (SPPU), Ganeshkhind Road, Pune 411007, Maharashtra, India., Ahire M; Institute of Bioinformatics and Biotechnology (IBB), Savitribai Phule Pune University, (SPPU), Ganeshkhind Road, Pune 411007, Maharashtra, India., Zinjarde SS; Institute of Bioinformatics and Biotechnology (IBB), Savitribai Phule Pune University, (SPPU), Ganeshkhind Road, Pune 411007, Maharashtra, India. Electronic address: smita@unipune.ac.in., Tamhane VA; Institute of Bioinformatics and Biotechnology (IBB), Savitribai Phule Pune University, (SPPU), Ganeshkhind Road, Pune 411007, Maharashtra, India. Electronic address: vatamhane@unipune.ac.in.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2018 Jan; Vol. 147, pp. 612-621. Date of Electronic Publication: 2017 Oct 10.
DOI: 10.1016/j.ecoenv.2017.09.028
Abstrakt: The present study deals with glutathione-S-transferase (GST) based detoxification of pesticides in Helicoverpa armigera and its potential application in eliminating pesticides from the environment. Dietary exposure of a pesticide mixture (organophosphates - chlorpyrifos and dichlorvos, pyrethroid - cypermethrin; 2-15ppm each) to H. armigera larvae resulted in a dose dependant up-regulation of GST activity and gene expression. A variant GST from H. armigera (HaGST-8) was isolated from larvae fed with 10ppm pesticide mixture and it was recombinantly expressed in yeast (Pichia pastoris HaGST-8). HaGST-8 had a molecular mass of 29kDa and was most active at pH 9 at 30°C. GC-MS and LC-HRMS analysis validated that HaGST-8 was effective in eliminating organophosphate type of pesticides and partially reduced the cypermethrin content (53%) from aqueous solutions. Unlike the untransformed yeast, P. pastoris HaGST-8 grew efficiently in media supplemented with pesticide mixtures (200 and 400ppm each pesticide) signifying the detoxification ability of HaGST-8. The amino acid sequence of HaGST-8 and the already reported sequence of HaGST-7 had just 2 mismatches. The studies on molecular interaction strengths revealed that HaGST-8 had stronger binding affinities with organophosphate, pyrethroid, organochloride, carbamate and neonicotinoid type of pesticides. The abilities of recombinant HaGST-8 to eliminate pesticides and P. pastoris HaGST-8 to grow profusely in the presence of high level of pesticide content can be applied for removal of such residues from food, water resources and bioremediation.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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