The Transcription Factors COUP-TFI and COUP-TFII have Distinct Roles in Arealisation and GABAergic Interneuron Specification in the Early Human Fetal Telencephalon.

Autor:	Alzu'bi A; Institute of Neuroscience, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.; Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK., Lindsay SJ; Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK., Harkin LF; Institute of Neuroscience, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.; Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.; School of Healthcare Science, Manchester Metropolitan University, UK., McIntyre J; Institute of Neuroscience, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.; Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK., Lisgo SN; Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK., Clowry GJ; Institute of Neuroscience, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
Jazyk:	angličtina
Zdroj:	Cerebral cortex (New York, N.Y. : 1991) [Cereb Cortex] 2017 Oct 01; Vol. 27 (10), pp. 4971-4987.
DOI:	10.1093/cercor/bhx185
Abstrakt:	In human telencephalon at 8-12 postconceptional weeks, ribonucleic acid quantitative sequencing and immunohistochemistry revealed cortical chicken ovalbumin upstream promotor-transcription factor 1 (COUP-TFI) expression in a high ventro-posterior to low anterior gradient except for raised immunoreactivity in the anterior ventral pallium. Unlike in mouse, COUP-TFI and SP8 were extensively co-expressed in dorsal sensory neocortex and dorsal hippocampus whereas COUPTFI/COUPTFII co-expression defined ventral temporal cortex and ventral hippocampus. In the ganglionic eminences (GEs) COUP-TFI immunoreactivity demarcated the proliferative zones of caudal GE (CGE), dorsal medial GE (MGE), MGE/lateral GE (LGE) boundary, and ventral LGE whereas COUP-TFII was limited to ventral CGE and the MGE/LGE boundary. Co-labeling with gamma amino butyric acidergic interneuron markers revealed that COUP-TFI was expressed in subpopulations of either MGE-derived (SOX6+) or CGE-derived (calretinin+/SP8+) interneurons. COUP-TFII was mainly confined to CGE-derived interneurons. Twice as many GAD67+ cortical cells co-labeled for COUP-TFI than for COUP-TFII. A fifth of COUP-TFI cells also co-expressed COUP-TFII, and cells expressing either transcription factor followed posterior or anterio-lateral pathways into the cortex, therefore, a segregation of migration pathways according to COUP-TF expression as proposed in mouse was not observed. In cultures differentiated from isolated human cortical progenitors, many cells expressed either COUP-TF and 30% also co-expressed GABA, however no cells expressed NKX2.1. This suggests interneurons could be generated intracortically from progenitors expressing either COUP-TF. (© The Author 2017. Published by Oxford University Press.)
Databáze:	MEDLINE
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