Short-term hypoxia and vasa recta function in kidney slices.
| Autor: | Braun D; Institute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Germany., Dietze S; Institute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Germany., Pahlitzsch TMJ; Institute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Germany., Wennysia IC; Institute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Germany., Persson PB; Institute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Germany., Ludwig M; Institute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Germany., Patzak A; Institute of Vegetative Physiology, Charité-Universitätsmedizin Berlin, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | Clinical hemorheology and microcirculation [Clin Hemorheol Microcirc] 2017; Vol. 67 (3-4), pp. 475-484. |
| DOI: | 10.3233/CH-179230 |
| Abstrakt: | Background: Descending vasa recta (DVR) supply the inner part of outer renal medulla an area at risk for hypoxic damages. Objective: We hypothesize increased vasoreactivity after hypoxia/re-oxygenation (H/R) in DVR, which might contribute to the reduced medullary perfusion after an ischemic event. Methods: Live kidney slices (200μm) from SD rats were used for functional experiments. TUNEL assay and H&E staining were used to estimate slice viability. Kidney slices were treated with carbogen or hypoxia (1% O2) for 60 or 90 min and vasoreactivity to Ang II (10-7 M) was recorded by DIC microscopy after re-oxygenation with carbogen. Expression of NOS and NADPH enzymes mRNA were determined in iron-perfusion isolated VR. Results: Percentage of apoptotic cells increased in control and H/R after 90 min in the medulla. Ang II- induced constriction of DVR was reduced after 90 min in control (compared to 60 min), but not after H/R. NOS enzymes mRNA expression levels decreased over 90 min hypoxia. Conclusions: Increased reactivity of DVR to Ang II after H/R compared to control (90 min) suggest a role of DVR in renal ischemia/reperfusion injury. |
| Databáze: | MEDLINE |
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