Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP MS).

Autor: Vacchina V; UT2A, Hélioparc Pau-Pyrénées, 2 Avenue du President Angot, 64000, Pau, France. veronique.vacchina@univ-pau.fr., Bierla K; IPREM-LCABIE, Hélioparc Pau-Pyrénées, 2 Avenue du Président Angot, 64000, Pau, France., Szpunar J; IPREM-LCABIE, Hélioparc Pau-Pyrénées, 2 Avenue du Président Angot, 64000, Pau, France., Lobinski R; IPREM-LCABIE, Hélioparc Pau-Pyrénées, 2 Avenue du Président Angot, 64000, Pau, France.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1661, pp. 153-162.
DOI: 10.1007/978-1-4939-7258-6_11
Abstrakt: Selenium (Se) is an element readily absorbed during the intestinal tract and distributed in the body. In biological fluids, tissues, and animal products, Se is known to be present mainly in the form of a selenoamino-acid (selenomethionine (SeMet) or selenocysteine (SeCys)). Both amino-acids have different biological activities which justifies their discrimination. Here, we describe the method allowing the simultaneous determination of SeMet and SeCys in blood/plasma, animal tissues, milk, and eggs by two-dimensional Liquid Chromatography coupled to Inductively Coupled Plasma Mass Spectrometry (2D HPLC-ICP MS).
Databáze: MEDLINE
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